Citation: LIU Wei-Ping, LIN Jin-Qiong, DU Yan, QI Ming-Yue, LIANG Guang-Tie, YANG Na, LIU Da-Yu. Anti-Cancer Drug Testing Using Multi-Layer Paper-Supported Breast Cancer Tissue Array[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(4): 579-585. doi: 10.11895/j.issn.0253-3820.151025 shu

Anti-Cancer Drug Testing Using Multi-Layer Paper-Supported Breast Cancer Tissue Array

LIU Wei-Ping ¹ ,
LIN Jin-Qiong ¹ ,
DU Yan ¹ ,
QI Ming-Yue ¹ ,
LIANG Guang-Tie ^1,2 ,
YANG Na ^1,2 ,
LIU Da-Yu ^1,2,3,,

1.
¹ Department of Laboratory Medicine, Guangzhou First People's Hospital Affiliated Hospital of Guangzhou Medical University, Guangzhou 510180, China;
2.
² Clinical Molecular Medicine and Molecular Diagnosis Key Laboratory of Guangdong Province, Guangzhou 510180, China;
3.
³ Clinical Research Center, the Second Hospital Affiliated to Dalian Medical University, Dalian 116027, China

Corresponding author: LIU Da-Yu,
Received Date: 30 December 2015
Available Online: 5 February 2016
Fund Project: 本文系国家自然科学基金(Nos.81371649,81271730,81171418) (Nos.81371649,81271730,81171418)广州市医药卫生科技项目(Nos.20121A021002,20121A011035)资助 (Nos.20121A021002,20121A011035)

This paper describes a multi-layer paper-supported breast cancer tissue array. The paper-based microchip with arrayed hydrophilic spots was fabricated by wax printing. After cell seeding, the microchip was folded to form an artificial solid tumor, and the multilayer paper was covered by a layer of microporous membrane that mimics endothelial layer. After culturing the paper-supported breast cancer tissue for a certain period of time, the multi-layer device was unfold thus enabling detections of cell survival, proliferation, morphology and metabolism on different layers. Breast cancer cells cultured on paper had a survival rate >80% and aggregated into tumor spheroids. Intracellular lactic acid levels increased with the extension of culture time. Compared to two dimensional(2D) counterparts, the decreased fraction of S phase cell(15% vs 60%) was detected. The paper-supported breast cancer tissue showed a drug response mechanism similar to the tumor in vivo. Cancer cells within the tissue were less sensitive to doxorubicin and the IC₅₀ value detected was significantly higher than that obtained with 2D cultures(5μmol/L vs 1.14μmol/L). The multi-layer paper-supported breast cancer tissue array developed in this work featured simple tissue reconstruction and easy operation, thus providing an ideal tool for anti-cancer drug testing.
- Paper-based microfluidic chip,
- Breast cancer,
- Tissue microarray,
- Microenvironment,
- Anti-cancer drug
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