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Citation: ZHAO Xiao-Ya, WANG Yue-Fei, YANG Fan, JIANG Zhen-Zuo, WANG Yan, YANG Long, PAN Zan-Hong. Rapid Analysis of Seven Short-Chain Fatty Acids in Human Saliva by Gas Chromatography[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(7): 1009-1014. doi: 10.11895/j.issn.0253-3820.150960 shu

Rapid Analysis of Seven Short-Chain Fatty Acids in Human Saliva by Gas Chromatography

ZHAO Xiao-Ya ^1,2, ,
WANG Yue-Fei ^1,2 ,
YANG Fan ^1,2 ,
JIANG Zhen-Zuo ^1,2 ,
WANG Yan ^1,2 ,
YANG Long ^1,2 ,
PAN Zan-Hong ³

1.
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
2.
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China;
3.
Pharmacy Department, The Second People's Hospital of Tianjin, Tianjin 300192, China

Received Date: 5 December 2015
Revised Date: 27 March 2016

Fund Project: This work was supported by the National Science and Technology Major Projects for "Major New Drugs Innovation and Development" (No. 2015ZX09J15102-004-004)

Figures(3)

A gas chromatographic (GC) method was established for the simultaneous analysis of seven short-chain fatty acids (SCFAs) including acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid and hexanoic acid in human saliva. Salivary sample was diluted by ethanol solution by ratio of 1:1 (V/V), with 0.5% (V/V) hydrochloric acid in sample solution, then vortexed and centrifuged. The supernatent was subjected to GC analysis. The chromatographic separation was performed on a DB-FFAP capillary column (30 m×0.25 mm×0.25 μm) using flame ionization detector (FID). The quantification was achieved by internal standard method. The experimental results showed that an excellent correlation coefficient (R²≥0.999) was obtained for all the calibration curves of seven SCFAs. The limits of detection (LOD, S/N=3) and limits of quantitation (LOQ, S/N=10) were 0.060-0.198 μg/mL and 0.180-0.594 μg/mL, respectively. The average recoveries were 94.8%-109.7% with relative standard deviation (RSD)≤4.3% (n=6). The developed method is simple, rapid and accurate, and suitable for the simultaneous determination of seven SCFAs in human saliva.
- Gas chromatography,
- Saliva,
- Short-chain fatty acids,
- Gut microbiota
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