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Citation: QIAN Zhuo-Zhen, LUO Dong-Lian, LUO Fang-Fang, YE Mei, TANG Shui-Fen. Determination of Peptide Antibiotics Residues in Sediment From Aquaculture Environment by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(6): 870-875. doi: 10.11895/j.issn.0253-3820.150838 shu

Determination of Peptide Antibiotics Residues in Sediment From Aquaculture Environment by High Performance Liquid Chromatography-Tandem Mass Spectrometry

1.
College of Materials, Xiamen University, Xiamen 361005, China;
2.
Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China

Received Date: 20 October 2015
Revised Date: 4 March 2016

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A new method for the determination of peptide antibiotics in sediment from aquaculture environment by high performance liquid chromatography-tandem mass spectrometry was developed. The target analytes in sediments were ultrasonically extracted twice with citrate buffer solution and methol mixture (3:4, V/V), followed by complexation with 0.5 g of Na₂EDTA, purification with 5 mL of methyl isobutyl ketone, and clean-up with HLB-SPE column. The analytes were separated on a MGII C₁₈ column by gradient elution with 0.1% formaic acid-0.1% formaic acid acetonitrile as mobile phase, detected in multiple reaction monitoring (MRM) with electrospray ionization (ESI) under positive ion mode, and quantified by external standard method. The calibration curves were linear (R²>0.999) over a concentration range of 10-10000 μg/L for colistin and bacitracin and 4-4000 μg/L for virginiamycin M₁. The limits of detection (S/N＝3) were 5 μg/kg for colistin and bacitracin and 2 μg/kg for virginiamycin M₁. The limits of quantification (S/N＝10) was 10 μg/kg for colistin and bacitracin and 4 μg/kg for virginiamycin M₁. At three spiked levels, the recoveries ranged from 79.7% to 91.6% (RSD=1.9%-10.8%), showing high sensitivity, good reproducibility and wide applicability.
- High performance liquid chromatography-tandem mass spectrometry,
- Aquaculture environment,
- Sediment,
- Peptide antibiotics,
- Residues
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1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Determination of Peptide Antibiotics Residues in Sediment From Aquaculture Environment by High Performance Liquid Chromatography-Tandem Mass Spectrometry

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

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通讯作者: 陈斌, bchen63@163.com