Citation: YANG Shan-Shan, YANG Ya-Nan, LI Xue-Lin, ZHANG Yan. Determination of Biogenic Amines in Cheese by On-line Solid Phase Extraction Coupled with Capillary High Performance Liquid Chromatography[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(3): 396-402. doi: 10.11895/j.issn.0253-3820.150811 shu

Determination of Biogenic Amines in Cheese by On-line Solid Phase Extraction Coupled with Capillary High Performance Liquid Chromatography

1.
Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China

Corresponding author: ZHANG Yan,
Received Date: 15 October 2015
Available Online: 23 November 2015
Fund Project: 本文系国家高技术研究发展计划基金资助项目(863计划,No.2011AA100807) (863计划,No.2011AA100807)

An on-line solid phase extraction coupled with capillary HPLC method was established for the simultaneous determination of fifteen kinds of biologic amines in cheese. The biogenic amines were concentrated on the solid phase extraction column, and transferred by the six-way valve to analytical column for separation and detection. Separation conditions on capillary HPLC, composition of on-line SPE mobile phase, pH of the sample solution and switching time of six-way switching valve were investigated to get better separation conditions of 15 biogenic amines. Optimum on-line SPE conditions including 5% of the acetonitrile-water as mobile phase for SPE column, pH=11 of the sample solution and 3 min of valve switching time were employed in the analytical method. The linear range of standard curve for fifteen biogenic amines was 0.25-50.0 mg/L; LOD were within the range of 0.05-0.25 mg/L. At spiked levels of 1, 20, 40 mg/kg, the recoveries of fifteen biogenic amines on four kinds of cheese ranged from 79.6% to 118.7% except methylamine, ethylamine, 3-methylbutanamine and 5-hydroxytryptamine; with RSDs from 0.3% to 14.9% except 3-methylbutanamine and 5-hydroxy-tryptamine. The method is accurate and reliable, and can be used to detect biogenic amines in cheese.
- On-line solid phase extraction,
- Capillary high performance liquid chromatography,
- Biogenic amines,
- Cheese
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