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Citation: ZHANG He, JIANG Bin, FENG Zhi-Biao, QU Yu-Xiao, LI Xuan. Separation of α-Lactalbumin and β-Lactoglobulin in Whey Protein Isolate by Aqueous Two-phase System of Polymer/Phosphate[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(5): 754-759. doi: 10.11895/j.issn.0253-3820.150800 shu

Separation of α-Lactalbumin and β-Lactoglobulin in Whey Protein Isolate by Aqueous Two-phase System of Polymer/Phosphate

Department of Applied Chemistry, Northeast Agricultural University, Harbin 150030, China

Received Date: 12 October 2015
Revised Date: 12 January 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 31201366)

Poly (ethylene glycol-ran-propylene glycol) monobutyl ether/phosphate aqueous two-phase system (ATPS) coupled with high performance liquid chromatography (HPLC) was developed for the separation of α-lactalbumin (α-LA) and β-lactoglobulin (β-LG) in whey protein isolate (WPI). The main parameters relating to this method were investigated and optimized. The effects of pH value, volume ratio of polymer and KH₂PO₄ solutions, concentration of NaCl and WPI on the partition of α-LA and β-LG in WPI were investigated. The results showed that efficient separation of both proteins in WPI could be achieved by using the ATPS with 4 mL poly (ethylene glycol-ran-propylene glycol) monobutyl ether solution (40%, m/m) and 4 mL KH₂PO₄ solution (15.5%, m/m), 0.40 g/10 mL NaCl, while the concentration of WPI was 1 mg/mL, at pH 4.0. Under the optimized conditions, α-LA was separated to the upper phase while β-LG to the bottom phase with the yield of 98.2% and 96.6% respectivelon
- Aqueous two-phase system,
- Whey protein isolate,
- Poly (ethylene glycol-ran-propylene glycol) monobutyl ether,
- Separation
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