Citation: XIA Qun, XIN Pei-Yong, CHU Jin-Fang. An Automated On-line Solid Phase Extraction-Liquid Chromatography-Tandem Mass Spectrometric Method for Six Endogenous Phytohormones Analysis in Rice[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(3): 409-415. doi: 10.11895/j.issn.0253-3820.150769 shu

An Automated On-line Solid Phase Extraction-Liquid Chromatography-Tandem Mass Spectrometric Method for Six Endogenous Phytohormones Analysis in Rice

XIA Qun ^1,2 ,
XIN Pei-Yong ¹ ,
CHU Jin-Fang ^1,,

1.
¹ National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
2.
² University of Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: CHU Jin-Fang,
Received Date: 29 September 2015
Available Online: 30 December 2015
Fund Project: 本文系国家自然科学基金(No.91417314) (No.91417314)

An automated on-line solid phase extraction-liquid chromatography-tandem mass spectrometry (on-line SPE-LC-MS/MS) method for the simultaneous quantification of six endogenous phytohormones including abscisic acid (ABA), indole-3-acetic acid (IAA), salicylic acid (SA), jasmonic acid (JA), indole-3-propionic acid (IPA) and indole-3-butyric acid (IBA) in rice tissues was described. Plant samples were extracted with methanol and on-line SPE procedure was performed with C₁₈ SPE cartridges. Analytes were eluted to C₁₈ analytical column with mobile phase and further analyzed by LC-MS/MS. The performance of the method was fully validated. Linearity showed good correlation (R²≥0.99). Limits of detections (S/N=3) were in the range of 0.1-0.8 μg／kg. Recoveries varied between 71.2% and 126%. The method was rapid and sensitive to determine multiple phytohormones in rice young panicles and the results were cross-validated with the off-line SPE-LC-MS/MS phytohormone analytical method. Finally, the method was applied to monitor the changes of ABA, IAA, SA and JA in wounded rice leaves. The trends were in accord with plant physiological processes.
- Phytohormones,
- On-line solid phase extraction-liquid chromatography-tandem mass spectrometry,
- Quantitative analysis,
- Rice
1. [1]
  1 Davies P J. Plant Hormones: Biosynthesis, Signal Transduction, Action. Springer, 2010
2. [2]
  2 WU Qian, WANG Lu, WU Da-Peng, DUAN Chun-Feng, GUAN Ya-Feng. Chinese Journal of Chromatography, 2014, 32(4): 319-329吴倩, 王璐, 吴大朋, 段春凤, 关亚风. 色谱, 2014, 32(4): 319-329
3. [3]
  3 CHEN Xia-Di, LU Qiao-Mei, CHENG Jin-Tian, ZHANG Lan. Chinese J. Anal. Chem., 2015, 43(1): 127-131陈吓俤, 卢巧梅, 程锦添, 张兰. 分析化学, 2015, 43(1): 127-131
4. [4]
  4 ZHONG Dong-Lian, DING Ming, TANG Fu-Bin, MO Run-Hong, TENG Ying. Chinese J. Anal. Chem., 2013, 41(11): 1739-1743钟冬莲, 丁明, 汤富彬, 莫润宏, 滕莹. 分析化学, 2013, 41(11): 1739-1743
5. [5]
  5 CAO Zhao-Yun, MA You-Ning, CHEN Ming-Xue, MOU Ren-Xiang, HU Xiu-Fang. J. Instrum. Anal., 2011, 30(12): 1419-1424曹赵云, 马有宁, 陈铭学, 牟仁祥, 胡秀芳. 分析测试学报, 2011, 30(12): 1419-1424
6. [6]
  6 Pan X Q, Welti R, Wang X M. Nat. Protocols, 2010, 5(6): 986-992
7. [7]
  7 Pan X Q, Welti R, Wang X M. Phytochemistry, 2008, 69(8): 1773-1781
8. [8]
  8 Fu J H, Chu J F, Sun X H, Wang J D, Yan C Y. Anal. Sci., 2012, 28(11): 1081-1087
9. [9]
  9 GU Xin, WU Jian-Ping, ZHANG Xin, LI Dan-Ni, YAN Feng, ZHOU Yue-Rong. Chinese J. Anal. Chem., 2014, 42(12): 1759-1766顾欣, 吴剑平, 张鑫, 李丹妮, 严凤, 周悦榕. 分析化学, 2014, 42(12): 1759-1766
10. [10]
  10 GUO Jian, YANG Xin-Lei, YE Ming-Li. Chinese J. Anal. Chem., 2011, 39(8): 1256-1260郭坚, 杨新磊, 叶明立. 分析化学, 2011, 39(8): 1256-1260
11. [11]
  11 JIA Peng-Yu, ZENG Ming-Fei, FENG Nai-Jie, ZHENG Dian-Feng, SUN Fu-Dong, SUN Rui, LI Zhao-Yang. Chinese J. Anal. Chem., 2014, 42(12): 1743-1749贾鹏宇, 曾明飞, 冯乃杰, 郑殿峰, 孙福东, 孙蕊, 李朝阳. 分析化学, 2014, 42(12): 1743-1749
12. [12]
  12 Zhong Q S, Qiu X X, Lin C Y, Shen L L, Huo Y, Zhan S, Yao J T, Huang T H, Kawano S, Hashi Y, Xiao L T, Zhou T. J. Chromatogr. A, 2014, 1359: 131-139
13. [13]
  13 A Jia-La-Tie, XUE Da-Wei, LI Shi-Gui, QIAN Qian, GUO Long-Biao. China Rice, 2006, 5: 1-3阿加拉铁, 薛大伟, 李仕贵, 钱前, 郭龙彪. 中国稻米, 2006, 5: 1-3
14. [14]
  14 Cai B D, Yin J, Hao Y H, Li Y N, Yuan B F, Feng Y Q. J. Chromatogr. A, 2015, 1406: 78-86
15. [15]
  15 Wang B, Chu J F, Yu T Y, Xu Q, Sun X H, Yuan J, Xiong G S, Wang G D, Wang Y H, Li J Y. Proc. Natl. Acad. Sci. U. S. A., 2015, 112(15): 4821-4826
16. [16]
  16 Zhang J H, Jia W S, Yang J C, Ismail A M. Field Crops Res., 2006, 97(1): 111-119
17. [17]
  17 Boatwright J L, Pajerowska-Mukhtar K. Mol. Plant Pathol., 2013, 14(6): 623-634
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