Citation: KONG Guang-Hui, LI Yong, PANG Tao, SHI Jun-Li. Quantitation of 18 Polyphenols in Tobacco Leaf Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2015, 43(12): 1913-1919. doi: 10.11895/j.issn.0253-3820.150706 shu

Quantitation of 18 Polyphenols in Tobacco Leaf Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

1.
Yunnan Academy of Tobacco Agricultural Science, Yuxi 653100, China

Corresponding author: LI Yong,
Received Date: 6 September 2015
Available Online: 21 October 2015
Fund Project: 本文系云南省烟草专卖局基金资助项目(No.2014YN11) (No.2014YN11)

Polyphenols are very important secondary metabolites for tobacco plants. They are also considered as the important flavor precursors for cigarette industry. A liquid chromatography-tandem mass spectrometry(LC-MS/MS)-based method was established for the simultaneous determination of 18 polyphenols in tobacco leaf. The developed method can be used to determine 15 more polyphenols compared to the standard method for industry(YC/T 202-2006). Different kinds of extraction solution were compared and a solution of methanol-water-chloroform(5:2:2, V/V) was selected for the extraction because of its highest yield. Method validation was performed and the result showed that all the 18 polyphenols have their linear correlation coefficients(R²) more than 0.99. The low limit of determination and the low limit of quantitation were in the range of 1-150μg/L and 6-350μg/L, respectively. Intra-day and Inter-day reproducibility were in the range of 2.4%-6.5% and 4.5%-10.1%, respectively. Recoveries were in the range of 89.5%-103.7%. The established method was then successfully used to analyze polyphenols of tobacco leaves planted under different environment temperatures. The polyphenols can be classified in four groups based on their response to the temperature changing:the concentration increase with the increasing environment temperature, the concentration decrease with the increasing environment temperature, the concentration decrease with the environment temperature changes from the normal set point, and the concentration does not change significantly with the changing environment temperature.
- Tobacco,
- Polyphenols,
- Ultra performance liquid chromatography,
- Tandem mass spectrometry
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