Citation: WANG Zeng-Huan, WANG Xu-Nuo, LIN Qin, CHEN Cheng-Tong, CHEN Ying-Na. Bioaccessibility of Microamount Metal Elements in Oyster and Cam Samples Using in vitro Digestion[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(2): 247-251. doi: 10.11895/j.issn.0253-3820.150655 shu

Bioaccessibility of Microamount Metal Elements in Oyster and Cam Samples Using in vitro Digestion

1.
Key Lab. of Fishery Ecology Environment, Guangdong Province; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences; Key Lab. of Aquatic Product Processing, Ministry of Agriculture, Guangzhou 510300, China

Corresponding author: WANG Zeng-Huan,
Received Date: 18 August 2015
Available Online: 16 October 2015
Fund Project: 本文系国家科技支撑计划课题(No.2009BADB7B02) (No.2009BADB7B02)农业部水产品加工重点实验室开放基金(No. NYJG201306) (No. NYJG201306)中央级科研院所基本科研业务费专项资金(Nos.2013YD08, 2015TS16)资助 (Nos.2013YD08, 2015TS16)

A bionic method, in vitro digestion, was used for the pretreatment of oyster and clam samples. The oyster and clam samples were digested at 37℃ under the action of bionic digestive juice including saliva, gastric juice and intestinal juice. The contents of trace metals in in vitro digestion extracts (gastric juice extracts and intestinal juice extracts) were determined by atomic absorption spectrometry. The bioaccessibility of trace metals was assessed using the contents of trace metals in bionic extracts. The results showed that the contents of Fe, Cu, Zn and Cd in gastric juice extracts of in vitro digestion on oysters were higher than those in intestinal juice extracts. The contents of Fe and Cu in gastric juice extracts of in vitro digestion on clam were not obviously different with that of in intestinal juice extracts. Otherwise, the contents of Zn and Cd in gastric juice extracts were much higher than those of in intestinal juice extracts. The bioaccessibility of Fe, Cu, Zn and Cd in oysters using in vitro digestion was 60.2%, 83.6%, 83.1% and 76.8%, respectively. The bioaccessibility of Fe, Cu, Zn and Cd in clams was 46.3%, 86.3%, 85.3% and 87.7%, respectively. The results will provide a scientific basis for further researches such as bioaccumulation and bioavailability of trace metal in oysters and clams, evaluation of the health risk of Cd from oyster and clam consumption.
- In vitro digestion,
- Bioaccessibility,
- Atomic spectrometry,
- Microamount metal,
- oyster and clam
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