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Citation: CHU Ya-Nan, ZHANG Jie-Yu, FENG Li-Ying, ZHOU Guo-Hua. Establishment of A Rapid and Inexpensive Identification Method for HLA-B^*58:01 Genotype[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(5): 693-697. doi: 10.11895/j.issn.0253-3820.150643 shu

Establishment of A Rapid and Inexpensive Identification Method for HLA-B^*58:01 Genotype

Department of Pharmacology, Jinling Hospital, Nanjing 210002, China

Received Date: 13 August 2015
Revised Date: 12 January 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21275161, 31300704)

A new method for polymorphism detection of rs9263726 site on Psoriasis Susceptibility1 Candidate1(PSORS1C1) was established based on the pyrosequencing technology and the improved method of genomic DNA extraction from whole blood. The detection limit of the method was 0.4 ng/μL genomic DNA. A total of 20 samples were detected by pyrosequencing, and the results were consistent with those of Sanger sequencing. A total of 683 clinical samples were analyzed with pyrosequencing method. The polymorphism distribution of rs9263726 in Chinese populations was summarized as GG (87.6%), GA (11.7%) and AA (0.7%). 46 clinical samples were detected to analyze the relationship between rs9263726 genotype and human leukocyte antigen gene HLA-B^*58:01 genotype. The results showed that the genotype of HLA-B^*58:01 could be predicted by polymorphism detection of rs9263726 site sequence. The sensitivity of the proposed method was 100%, and the specificity was 91.3%. This study demonstrated a new method for HLA-B^*58:01 genotyping.
- Human leukocyte antigen gene HLA-B^*58:01,
- Pyrosequencing,
- Rs9263726 site,
- Genotype
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Establishment of A Rapid and Inexpensive Identification Method for HLA-B*58:01 Genotype

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

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通讯作者: 陈斌, bchen63@163.com

Establishment of A Rapid and Inexpensive Identification Method for HLA-B^*58:01 Genotype