Citation: LI Zhe-Yu, SUN Kai, ZHANG Xiao-Yan, LIU Shao-Qin, JIANG Lei, REN Nan-Qi. Advance in Microfluidic Devices for Fractionation of DNA Fragments[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(4): 569-578. doi: 10.11895/j.issn.0253-3820.150589 shu

Advance in Microfluidic Devices for Fractionation of DNA Fragments

1.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China

Corresponding author: SUN Kai,
Received Date: 23 July 2015
Available Online: 28 September 2015
Fund Project: 本文系城市水资源与水环境国家重点实验室开放基金(No.2011TS02)资助 (No.2011TS02)

Current next generation sequencing works faster and the price is coming down. However, the workflows have numerous manual processes, which contribute to bottleneck and process inefficiency. One of the most time consuming steps is electrophoretic gel-based fractionation of a large number of fragments of interest from the library generation process. In recent years, several instruments were first introduced into the market, and then were tested soon by many famous DNA sequencing centers and platforms. This paper introduces the development of DNA fractionation techniques including capillary electrophoresis and microfluidic devices. Moreover, our argument raises the bottleneck in fractionating DNA fragments on the chips. Finally, we provide insights into the challenges of DNA fractionation and perspectives.
- Capillary electrophoresis,
- Microfluidic chip,
- Fractionation,
- DNA sequencing,
- Gene expression,
- Review
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沈阳化工大学材料科学与工程学院沈阳 110142