Citation: YI Yong, TANG Xu, LIN Xi-Huang, LIU Yuan-Sen, LIN Ling, XU Chang-An. Simultaneous Determination of 4 Kind of Plant Hormones in Sargassum by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(1): 124-130. doi: 10.11895/j.issn.0253-3820.150585 shu

Simultaneous Determination of 4 Kind of Plant Hormones in Sargassum by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry

YI Yong ^1,2 ,
TANG Xu ¹ ,
LIN Xi-Huang ¹ ,
LIU Yuan-Sen ¹ ,
LIN Ling ¹ ,
XU Chang-An ^1,,

1.
¹ Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China;
2.
² College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Corresponding author: XU Chang-An,
Received Date: 22 July 2015
Available Online: 18 September 2015
Fund Project: 本文系海洋公益性行业专项(No.201405038) (No.201405038)厦门南方海洋中心专项(No.14GZP004NF04) (No.14GZP004NF04)国家海洋局第三海洋研究所基本科研业务费(海三科2013005)资助 (海三科2013005)

A method of simultaneous determination of four kind of auxins (Indole-3-acetic acid, Indole-3-butyric acid, Abscisic acid, Zeatin) in sargassum by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was established. After extracted by 70% methanol, the samples were enriched by the PCX and PAX solid-phase column. Then the samples were purified by reversed phase C₁₈ column using 5 mmol/L ammonium acetate (containing 0.01% formic acid) and methanol as a mobile phase in gradient elution. On the base of multiple reaction monitoring (MRM) analysis, the results showed that the correlation coefficient for all auxins examined exceeded 0.9990 in the range of 0.01-1.0 μg/mL. The recovery was from 84.3% to 102.1%, respectively. The relative standard deviation was less than 10%. Besides, the detection limit of this method was from 0.025 μg/kg to 0.2 μg/kg.
- Sargassum,
- Double columns combination,
- Ultra performance liquid chromatography tandem mass spectrometry,
- Endogenous plant hormones
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