Citation: XIA Chang, HAI Xin, CHEN Shuai, CHEN Xu-Wei, WANG Jian-Hua. Preparation of Graphene Quantum Dots/Ag Nanoparticles Nanocomposites for Colorimetric Detection of H₂O₂ and Glucose[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(1): 41-48. doi: 10.11895/j.issn.0253-3820.150568 shu

Preparation of Graphene Quantum Dots/Ag Nanoparticles Nanocomposites for Colorimetric Detection of H₂O₂ and Glucose

XIA Chang ¹ ,
HAI Xin ¹ ,
CHEN Shuai ² ,
CHEN Xu-Wei ^1,, ,
WANG Jian-Hua ^1,,

1.
¹ Research Center for Analytical Sciences, College of Sciences, Northeastern University, Shenyang 110819, China;
2.
² College of Life and Health Science, Northeastern University, Shenyang 110819, China

Corresponding author: CHEN Xu-Wei, WANG Jian-Hua,
Received Date: 16 July 2015
Available Online: 13 August 2015
Fund Project: 本文系国家自然科学基金项目(Nos.21275027,21235001,21475017)资助 (Nos.21275027,21235001,21475017)

Graphene quantum dots/Ag nanoparticles nanocomposite (GQDs/AgNPs) was prepared via in-situ growth of AgNPs on the surface of GQDs, in which GQDs served as both reducing agent and stabilizer. The as-prepared nanocomposites were mono-dispersed with an average diameter of less than 30 nm. The obtained GQDs@AgNPs nanocomposites exhibited excellent intrinsic peroxidese-like activity, which could catalyze the oxidization of 3,3',5,5'-tetramethylbenzidine (TMB) by H₂O₂ to produce a colour product. Steady-state kinetic assays showed that the catalytic activity of GQDs/AgNPs towards H₂O₂ fitted well with typical Michaelis-Menten kinetic model, followed by the ping-pong mechanism. Compared with the horseradish peroxidase (HRP), GQDs/AgNPs showed higher affinity towards TMB and H₂O₂ substrate. Based on the intrinsic peroxidese-like activity of GQDs/AgNPs nanocomposites and the production of H₂O₂ after the oxidation of glucose, a colorimetric method was developed for the detection of H₂O₂ and glucose, along with detection limit of 0.18 μmol/L and 1.6 μmol/L, respectively. The present method was applied to the detection of glucose in human serum, and the obtained results agreed with that obtained by reference method.
- Graphene quantum dots/Ag nanopartides nanocomposite,
- Peroxidase-like activity,
- Hydrogen peroxide,
- Glucose
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Preparation of Graphene Quantum Dots/Ag Nanoparticles Nanocomposites for Colorimetric Detection of H₂O₂ and Glucose