Citation: ZHU Jing, LI Yan, YONG Li, ZHENG Bo, ZOU Xiao-Li, LIU Zhen. Determination of Metabolites of Benzene Compounds and Trichloroethylene in Urinary by Isotope Dilution-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(1): 81-87. doi: 10.11895/j.issn.0253-3820.150497 shu

Determination of Metabolites of Benzene Compounds and Trichloroethylene in Urinary by Isotope Dilution-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

ZHU Jing ¹ ,
LI Yan ¹ ,
YONG Li ² ,
ZHENG Bo ¹ ,
ZOU Xiao-Li ^1,, ,
LIU Zhen ³

1.
¹ Department of Laboratory Technology and Science of Public Health, West China School of Public Health, Sichuan University, Chengdu 610041, China;
2.
² Center for Disease Control and Prevention of Sichuan Province, Chengdu 610041, China;
3.
³ National Center for Birth Defect Monitoring, West China Second University Hospital, Sichuan University, Chengdu 610041, China

Corresponding author: ZOU Xiao-Li,
Received Date: 17 June 2015
Available Online: 21 October 2015
Fund Project: 本文系国家重点基础研究发展计划(No.2010CB529502)资助 (No.2010CB529502)

A method for simultaneous determination of metabolites of benzene compounds and trichloroethylene in pregnant urine by isotope dilution-ultra performance liquid chromatography-tandem mass spectrometry was established. Urine samples were diluted 10 times with ammonium acetate buffer containing working mixed internal standard and the filtrate was injected into UPLC/MS/MS for analysis. UPLC-MS/MS was performed on Waters C₁₈ column and the gradient elution was used for separation. The negative electrospray ionization (ESI) source under the multiple reaction monitoring (MRM) mode was used for quantitative analysis. The limits of detection (LOD) ranged from 0.0350 μg/L to 1.75 μg/L. The inter-day and intra-day precisions of method were 1.2%-9.1% and 2.0%-9.7%, respectively. The proposed method was applied on detecting 650 urine samples of pregnant women in different region and the results were analyzed by statistical method. The spiked recoveries were in the range of 85.0%-104%. The method was quick, sensitive and suitable for batch analysis of urine samples.
- Isotope dilution,
- Ultra performance liquid chromatography-tandem mass spectrometry,
- Benzene compounds,
- Trichloroethylene,
- Metabolite,
- Urinary
1. [1]
  1 CHEN Wen, XU Lei, DENG Li-Xia, LI Fang-Hong, YANG Xing-Fen, ZHENG Chang-Liang, CHEN Gang. Chin. Occup. Med., 2000,27(5):9-11 陈雯, 徐雷, 邓丽霞, 李方红, 杨杏芬, 郑创亮, 陈刚.中国职业医学,2000,27(5):9-11
2. [2]
  2 LI Xing-Hu, WANG Jin-Feng, ZHENG Xiao-Ying, ZHANG Ke-Li. Chin. J. Public Health, 2005,21(10):1158-1160 李新虎, 王劲峰, 郑晓瑛, 张科利.中国公共卫生,2005,21(10):1158-1160
3. [3]
  3 Boogarrd P J, Van Sittert N J. Environ. Health Perspect, 1996,104(6):1151-1157
4. [4]
  4 Qu Q, Melikian A A, Li G, Shore R, Chen L, Cohen B, Yin S, Kagan M R, Li H, Meng M, Jin X, Winnik W, Li Y, Mu R, Li K. Am. J. Ind. Med., 2000,37(5):522-531
5. [5]
  5 Inoue O,Kanno E, Yusa T, Kakizaki M, Watanabe T, Higashikawa K, Ikeda M. Biomarkers, 2001,6(3):190-203
6. [6]
  6 Laffon B, Lema M, Mendez J. J. Chromatogr.B, 2001,753(2):385-393
7. [7]
  7 Schad H, Schafer F, Weber L, Seidel H J. J. Chromatogr. A, 1992,593(1-2):147-151
8. [8]
  8 LIU Yun-Fu, TAN Guang-Hui. Chinese Journal of Health Laboratory Technology, 2013,23(3):625-627 刘云富, 谭广辉.中国卫生检验杂志,2013,23(3):625-627
9. [9]
  9 DU Hong, KONG Yu-Mei, WANG Ping, DU Guang-Yu. The Administration and Technique of Environmental Monitoring, 2000,12(6):33-34 杜宏, 孔玉梅, 王平, 杜广玉.环境监测管理与技术,2000,12(6):33-34
10. [10]
  10 LIU Ming-Ren, YANG Xue-Yu, WANG Li, ZHANG Zhao-Liang. Environmental Science and Technology, 2011,34(11):135-138 刘明仁, 杨学雨, 王力, 张昭良.环境科学与技术,2011,34(11):135-138
11. [11]
  11 LIU Wen-Jun, DUAN Ai-Ling, GAO Lan-Zhen. Gansu Environmental Study and Monitoring, 2000,13(4):195-197 刘文君, 段爱玲, 高兰珍.甘肃环境研究与监测,2000,13(4):195-197
12. [12]
  12 SUN Chen-Jun. Biomaterials Inspection,Beijing:People's Medical Publishing House, 2006:193 孙成均主编.生物材料检验,北京:人民卫生出版社, 2006:193
13. [13]
  13 Sterz K, Kohler D, Schettgen T, Scherer G. J. Chromatogr. B, 2010,878(27):2502-2505
14. [14]
  14 van Eeckhaut A, Lanckmans K, Sarre S, Smolders I, Michotte Y. J. Chromatogr. B, 2009,877(23):2198-2207
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