Citation: ZHOU Yao-Feng, XIONG Si-Cheng, JIANG Hu, DUAN Hong, XIONG Yong-Hua, WANG Andrew. Immunochromatographic Assay for Quantitative Detection of Ochratoxin A in Maize by Quantum Dot Beads[J]. Chinese Journal of Analytical Chemistry, ;2015, 43(12): 1837-1843. doi: 10.11895/j.issn.0253-3820.150468 shu

Immunochromatographic Assay for Quantitative Detection of Ochratoxin A in Maize by Quantum Dot Beads

ZHOU Yao-Feng ¹ ,
XIONG Si-Cheng ¹ ,
JIANG Hu ^1,2 ,
DUAN Hong ^1,2 ,
XIONG Yong-Hua ^1,2,, ,
WANG Andrew ³

1.
¹ State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;

Corresponding author: XIONG Yong-Hua,
Received Date: 5 June 2015
Available Online: 25 August 2015
Fund Project: 本文系国家重点基础研究发展计划(973计划,No.2013CB127804) (973计划,No.2013CB127804)国家自然科学基金(No.31271863) (No.31271863)江西省主要学科与技术带头人培养计划(No.2014BCB22004)资助 (No.2014BCB22004)

Fluorescence probes were prepared by coupling the ascetic fluid containing anti-ochratoxin A(OTA) monoclonal antibody with quantum dot beads(QBs) using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride method. The OTA-labeled bovine serum albumin(BSA) conjugates and donkey anti-mouse polyclonal antibodies were sprayed onto the nitrocellulose membrane as the test and control lines, respectively. The resultant fluorescence probes were introduced into the immunochromatographic strip(ICA) for sensitive, rapid and quantitative detection of OTA in maize based on the ratio of fluorescence intensities of test and control lines. The QBs-based ICA exhibited dynamic linear range for detection of OTA in maize extract from 0.05 ng/mL to 1.0 ng/mL(Y=0.259lnX+0.897, R²=0.995), with the median inhibitory concentration of(0.215±0.023) ng/mL(n=5). The detection limit for OTA was 0.05 ng/mL in maize extract, and the detection time of the proposed QBs-based ICA for each sample was 10 min. The recoveries of OTA at three spiked levels were 107.2%-125.5%, while relative standard deviations were below 10%. Forty natural maize samples were assayed using both QB-ICA and enzyme-linked immunosorbent assay, and the results from the two methods showed a highly significant correlation(R²=0.975).
- Quantum dots beads,
- Immunochromatographic assay,
- Ochratoxin A,
- Quantitative detection
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