Citation: XIE Hui-Hui, XIE Tong, XU Jian-Ya, SHEN Cun-Si, LAI Zi-Juan, XU Niu-Sheng, WANG Shou-Chuan, SHAN Jin-Jun. Metabolomics Study of Aconitine and Benzoylaconine Induced Reproductive Toxicity in BeWo Cell[J]. Chinese Journal of Analytical Chemistry, ;2015, 43(12): 1808-1813. doi: 10.11895/j.issn.0253-3820.150430 shu

Metabolomics Study of Aconitine and Benzoylaconine Induced Reproductive Toxicity in BeWo Cell

1.
¹ Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics in Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China;

Corresponding author: SHAN Jin-Jun,
Received Date: 23 May 2015
Available Online: 31 August 2015
Fund Project: 本文系国家自然科学基金(No.81303299) (No.81303299)江苏省研究生培养创新工程(No.SJZZ_0124)资助 (No.SJZZ_0124)

An intracellular metabonomics method based on gas chromatography coupled with mass spectrometer(GC-MS/MS) was established to explore the toxicity mechanism of aconitine and benzoylaconine. BeWo, the continuous cell lines originated from human placenta were selected to establish the in vitro placenta model. The intracellular metabolites were extracted after exposed with 5μg/mL aconitine and benzoylaconine for 0, 6, 12, 24 and 36 h, respectively. The intracellular metabolic profiling was acquired by GC-MS/MS. Orthogonal partial least squares discriminant analysis(OPLS-DA) score plot showed the time-dependent change of the metabolomics profiles between the control and intervention groups. After screened by variable influence on projection(VIP) value, one-way analysis of variance and searched with NIST 2014 database, we identified 11 metabolites, including proline, glycine, threonine, glutamic acid, N-acetylglutamine, glutamine, lysine, histidine, succinic acid, glucose and galactose, which mainly involved these pathways:(1) glutamine and glutamate metabolism,(2) glycine, serine and threonine metabolism,(3) alanine, aspartate and glutamate metabolism,(4) lysine degradation,(5) arginine and proline metabolism and(6) histidine metabolism. The results demonstrated that amino acid metabolism was the main metabolic pathway and responsible for the placental and fetal toxicity of aconitine and benzoylaconine.
- Aconitine,
- Benzoylaconine,
- Gas chromatography-mass spectrometry,
- Metabonomics,
- BeWo cell,
- Fetal toxicity
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沈阳化工大学材料科学与工程学院沈阳 110142