Citation: LIAO Xiao-Qing, WANG Hui-Ying, LI Zai-Jun. Fluorescence “on-off” Response of Bovine Serum Albumin-Cu System Towards Hydrogen Peroxide and L-Cysteine and Their Analysis Applications[J]. Chinese Journal of Analytical Chemistry, ;2015, 43(12): 1820-1828. doi: 10.11895/j.issn.0253-3820.150413 shu

Fluorescence “on-off” Response of Bovine Serum Albumin-Cu System Towards Hydrogen Peroxide and L-Cysteine and Their Analysis Applications

1.
¹ School of Chemical and Materials Engineering, Jiangnan University, Wuxi 214122, China;

Corresponding author: LI Zai-Jun,
Received Date: 20 May 2015
Available Online: 3 July 2015
Fund Project: 本文系国家自然科学基金资助项目(No.21176101) (No.21176101)

The synthesis and application of copper nanoclusters(CuNCs) as optical probe have attracted a great attention. The study shows that bovine serum albumin(BSA) CAN react with copper ions(Cu²⁺) in a base medium to form stable BSA-Cu complex. In the solution, the introduction of hydrogen peroxide can remarkably accelerate the formation of CuNCs. At the same time, the fluorescence intensity rapidly increases. Based on the fluorescence "light-on" response of BSA-Cu system, a kinetics method was developed for the fluorescent detection of hydrogen peroxide. The fluorescence intensity of BSA-Cu linearly increased with the increase of hydrogen peroxide in the range from 1.0×10^-6 mol/L to 1.5×10^-3 mol/L with the detection limit of 3.1×10^-7 mol/L(S/N=3). After that, the collected BSA-Cu solution was placed until its fluorescence intensity increases to the maximum value, in which the Cu²⁺ ions were fully changed into CuNCs. The experiment demonstrated that the addition of L-cysteine into the solution led to an obvious fluorescence quenching. Based on the fluorescence "light-off" response of BSA-Cu system towards L-cysteine, an analytical method was established for the fluorescent determination of L-cysteine. The fluorescence intensity linearly reduced with the increase of L-cysteine concentration in the range of 2.0×10^-4-1.0×10^-2 mol/L with the detection limit of 5.7×10^-5 mol/L(S/N=3). Finally, the resulted BSA-Cu waste was treated by high temperature ashing and then dissolving with sulfuric acid, in which the CuNCs were turned into Cu²⁺ ions. The resulting Cu²⁺ solution continued to be used for the detection of H₂O₂ and L-cysteine in the next cycle. In the work, the cycle detection of hydrogen peroxide and L-cysteine and reuse of copper could be achieved using the conversion between Cu²⁺ and copper nanoclusters. The method provides the characteristics of high sensitivity, low cost and environment-friendly, and can be widely used for routine analysis of hydrogen peroxide and L-cysteine.
- Copper ions,
- Copper nanoclusters,
- Fluorescence probe,
- Hydrogen peroxide,
- L-Cysteine
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沈阳化工大学材料科学与工程学院沈阳 110142