Citation: ZHANG Sheng-Hu, ZHANG Yi-Xi, JI Gui-Xiang, XU Huai-Zhou, LIU Ji-Ning, SHI Li-Li. Determination of Bisphenol A, Tetrabromobisphenol A and 4-Tert-Octylphenol in Child and Adult Urine Samples Using High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(1): 19-24. doi: 10.11895/j.issn.0253-3820.150404 shu

Determination of Bisphenol A, Tetrabromobisphenol A and 4-Tert-Octylphenol in Child and Adult Urine Samples Using High Performance Liquid Chromatography-Tandem Mass Spectrometry

1.
¹ Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China;
2.
² College of Resources and Environmental Engineering, Guizhou University, Guiyang 550025, China

Corresponding author: SHI Li-Li,
Received Date: 18 May 2015
Available Online: 18 September 2015
Fund Project: 本文系国家高技术研究发展计划(863计划)资助项目(No.2013AA06A308) (863计划)资助项目(No.2013AA06A308)

A method using high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry for quantitative determination of bisphenol A (BPA), tetrabromobisphenol A (TBBPA) and 4-tert-octylphenol (OP) in urine samples of children and adults was developed. The frozen urinary samples were unfrozen at room temperature, extracted by liquid-liquid extraction, evaporated to dryness, and dissolved by methanol so as to be analyzed by LC-MS/MS. The calibration curves for the determination of the three target compounds were linear in the concentration range of 0.2-50 μg/L, correlation coefficients (R) were greater than 0.99, and the quantitative limits (LOQ) were 0.05-0.27 μg/L. The recoveries of different concentration levels (5, 10 and 50 μg/L) were in the range of 85.7%-118.6% and the relative standard deviation (RSD) was 4.1%-10.5%. This method was successfully applied to the determination of BPA, TBBPA and OP in urine samples from 20 groups of children and adults. The results showed that exposure concentrations of TBBPA and OP in urine samples of children and adults were basically same, but exposure levels of BPA had significant difference (children >adults (Father or Mother)).
- Bisphenol A,
- Tetrabromobisphenol A,
- 4-Tert-octylphenol,
- High performance liquid chromatography-tandem mass spectrometry,
- Children,
- Adults,
- Urine
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