Citation: LI Yuan, YUAN Guo-Lin, XIA Chun-Yong, YU Chao. Construction of a Cell Impedance Biosensor Based on Polypyrrole-Indium Tin Oxide Micro-Electrode for Detecting Cell Biology Behavior[J]. Chinese Journal of Analytical Chemistry, ;2015, 43(12): 1844-1850. doi: 10.11895/j.issn.0253-3820.150396 shu

Construction of a Cell Impedance Biosensor Based on Polypyrrole-Indium Tin Oxide Micro-Electrode for Detecting Cell Biology Behavior

LI Yuan ^1,2 ,
YUAN Guo-Lin ¹ ,
XIA Chun-Yong ¹ ,
YU Chao ^1,,

1.
¹ Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, China;

Corresponding author: YU Chao,
Received Date: 14 May 2015
Available Online: 25 August 2015
Fund Project: 本文系重庆市科委自然科学基金计划资助项目(cstc2012jjA10046) (cstc2012jjA10046)重庆市永川区创新能力建设平台项目(Ycstc,2014bf5001) (Ycstc,2014bf5001)重庆医科大学附属永川医院院级重点研究项目(YJZD201302)资助 (YJZD201302)

An indium tin oxide(ITO) microelectrode was fabricated by etching the insulating layer of photosensitive dry film using lithography technology, then polypyrrole(PPy) layer with different thickness was electrodeposited on the surface of the ITO microelectrode by electrochemical cyclic voltammetry to get PPy-ITO microelectrode. The effect of the thickness of PPy layer on the impedance characteristic of PPy-ITO microelectrode was examined by electrochemical impedance spectroscopy(EIS). The biocompatibility of the PPy-ITO microelectrodes was investigated by adhesion and proliferation experiment of human lung cancer cell A549. Finally, using PPy-ITO microelectrode as sensing electrode, biology information on the adhesion, proliferation and epithelial-mesenchymal transition(EMT) of A549 was tested and analyzed by EIS and equivalent circuit fitting. The results showed that the PPy-ITO microelectrode prepared under optimal parameter(electrodeposition for five cycles) had a lower electrical impedance and a better cell compatibility than bare ITO microelectrode. The changes of cytoplasm membrane capacitance, intercellular resistance and the gap resistance between cell and polypyrrole film during the processes of adhesion, proliferation and epithelial-mesenchymal transition(EMT) of A549 could be detected by a cell impedance biosensor based on the PPy-ITO microelectrode.
- Biosensor,
- Electrochemical impedance spectroscopy,
- Polypyrrole,
- Indium tin oxide
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