Citation: LIN Shi-Yao, CONG Ri-Lin, LI Qi, SUN Zhao-Xia, XU Wei, SHA Mei, YU Li-Shuang. Determination of Triptolide in Rat Synoviocytes by Microemulsion Electrokinetic Chromatography[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(1): 49-53. doi: 10.11895/j.issn.0253-3820.150393 shu

Determination of Triptolide in Rat Synoviocytes by Microemulsion Electrokinetic Chromatography

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College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China

Corresponding author: YU Li-Shuang,
Received Date: 13 June 2015
Available Online: 12 September 2015
Fund Project: 本文系由国家自然科学基金项目(No.81202913) (No.81202913)福建省科技厅社会发展重点项目(No.2013Y0057) (No.2013Y0057)福建省卫生系统中青年骨干人才培养项目(No.2014-ZQN-JC-27) (No.2014-ZQN-JC-27)福建中医药大学校管科研课题-重点学科专项(No.X2014133-学科)资助 (No.X2014133-学科)

A method was established for the determination of triptolide in rat synoviocytes by microemulsion electrokinetic chromatography (MEEKC). Synoviocytes were divided into control groups and treatment groups by the different administration time of triptolide, and the concentrations of triptolide in intercellular and extracellular fluid were determined. The determination of triptolide by MEEKC was performed at 25℃ under the conditions including running buffer of 5 mmol/L borate-10 mmol/L phosphate (96%, V/V, pH 9.0) with 1% SDS (w/V), 3% 1-butanol (V/V) and 1% ethyl acetate (V/V), 25 kV of running voltage, 3.45 kPa×6 s of inject pressure and 214 nm of test wavelength. The results showed that triptolide was baseline separation, and there was a good linearity for triptolide in intercellular and extracellular of synoviocytes and the correlation coefficients were 0.9995 and 0.9991, respectively. The concentrations of triptolide in intercellular and extracellular of synoviocytes were 0.736 μg/mL and 20.745 μg/mL, respectively. The present method is simple, accurate, sensitive and precise, and can be used to study dynamic change of the triptolide concentration in rat synoviocytes and provide methodology basis for further study the influence of effective compounds of TCM on synoviocytes' function and activity.
- Microemulsion electrokinetic chromatography,
- Synoviocytes,
- Triptolide
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沈阳化工大学材料科学与工程学院沈阳 110142