Citation: LI Ning-Bo, HE Feng-Yun, LI Li, HU Yao-Juan, BAI Cheng-Chao, DUAN Yu-Qing. Selective Determination of Uric Acid at Nitrogen-doped Graphene/Chitosan Composite Modified Electrode in the Presence of a Large Amount of Ascorbic Acid[J]. Chinese Journal of Analytical Chemistry, ;2015, 43(12): 1859-1863. doi: 10.11895/j.issn.0253-3820.150340 shu

Selective Determination of Uric Acid at Nitrogen-doped Graphene/Chitosan Composite Modified Electrode in the Presence of a Large Amount of Ascorbic Acid

1.
School of Environmental Science, Nanjing Xiaozhuang University, Nanjing 211171, China

Corresponding author: HE Feng-Yun,
Received Date: 27 April 2015
Available Online: 10 August 2015
Fund Project: 本文系南京大学生命分析国家重点实验室开放研究基金(No.SKLACLS1308) (No.SKLACLS1308)江苏省大学生创新训练项目(No.201411460025Y) (No.201411460025Y)

Nitrogen-doped graphene was synthesized by one-step hydrothermal method. A nitrogen-doped graphene-chitosan modified electrode(N-GN-CS/GCE) was prepared and the electrochemical behavior of uric acid in the presence of a large amount of ascorbic acid was investigated at the modified electrode. The peak current of uric acid at the modified electrode was 7 times as that at unmodified electrode. The peak separation of uric acid and ascorbic acid was 362 mV and uric acid could be accurately determined in the presence of a large amount of ascorbic acid. Some experimental parameters such as dopping volume, pH and scan rate were optimized and a standard curve was also established using differential pulse voltammetry. The linear range between the peak current and the concentration of uric acid was 0.1~20μmol/L and 20-400μmol/L, and the detection limit was estimated to be 0.01μmol/L. The proposed method was applied to the analysis of uric acid in human urine sample with recoveries between 99.7% and 103.4%.
- Nitrogen dopped graphene,
- Ascorbic acid,
- Uric acid
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沈阳化工大学材料科学与工程学院沈阳 110142