Citation: ZENG Han, YANG Yang, LI Xiao-Juan, BAI Xi. Performance of Laccase-entrapped Magnetic Nano-particle Composite Modified Electrode as Biocathode in Enzymatic Biofuel Cell and Elecrochemical Sensor for Oxygen Detection[J]. Chinese Journal of Analytical Chemistry, ;2015, 43(12): 1794-1800. doi: 10.11895/j.issn.0253-3820.150337 shu

Performance of Laccase-entrapped Magnetic Nano-particle Composite Modified Electrode as Biocathode in Enzymatic Biofuel Cell and Elecrochemical Sensor for Oxygen Detection

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Laboratory of New Energy Materials Chemistry, Chemistry and Chemical Engineering Academy, XinJiang Normal University, Urumuqi 830054, China

Corresponding author: ZENG Han,
Received Date: 26 April 2015
Available Online: 21 July 2015
Fund Project: 本文系国家自然科学基金(No.21363024) (No.21363024)新疆维吾尔自治区2013年度高校科研计划青年教师培育项目(No.XJEDU2013S29) (No.XJEDU2013S29)新疆师范大学博士科研启动基金项目(No.XJNUBS1228)资助 (No.XJNUBS1228)

A nano-composite of phthalated Chitosan and magnetic ferroferric oxide nano-particle was prepared by mechanically mixing as method, and laccase was then adsorbed on the composite ot obtain immobilized laccase. Then the composite with laccase was dripped onto the surface of glassy carbon electrode and dried in air under room temperature to prepare laccase-immobilized biocathode. Catalytic effect in oxygen reduction reaction and performance of oxygen electrochemical sensor of this laccase-based electrode were systematically investigated by cyclic voltammetry, linear scanning voltammetry and chronoamperometry. Experimental results indicated that direct electron transfer between enzyme active centre T1 in laccase and conductive matrix ccould be achieved in solution without any electron relay, characterized with one-electron quasi-reversible redox reaction signal(Equilibrium potential:798 mV, very close to the formal potential of T1 centre in laccase 780 mV). It also featured with surface coverage of 1.5×10^-9 mol/cm² conductive enzyme molecules, electron relay rate of 0.05 s^-1, onset potential for oxygen reduction of 930 mV, substrate turn-over frequency of 0.3 dioxygen molecules/s. Linear relationship in steady catalytic current of laccase-based electrode versus oxygen concentration was found within the oxygen concentration range of 2.6-33.9μmol/L under optimal conditions. This electrode as oxygen electrochemical sensor showed a low detection limit of 0.86μmol/L, high sensitivity(17.2μA·L/μmol), and Michaelis-Menten constant K_M=131.1μmol/L. Further tests confirmed its excellent reproducibility and long-term usability in catalytic function towards oxygen reduction, with optimal pH=4.4.
- Phthaloyl chitosan,
- Magnetic ferroferric oxide nanoparticle,
- Laccase,
- Biocathode in enzymatic biofuel cell,
- Electrochemical sensor
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沈阳化工大学材料科学与工程学院沈阳 110142