Citation: LIANG Shi-Zheng, PAN Jia-Rong, ZHANG Chi, FENG Tao, FU Li-Li. Simultaneous Detection of Ractopamine, Clenbuterol and Salbutamol by Suspension Array Technology[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(4): 640-646. doi: 10.11895/j.issn.0253-3820.150172 shu

Simultaneous Detection of Ractopamine, Clenbuterol and Salbutamol by Suspension Array Technology

LIANG Shi-Zheng ¹ ,
PAN Jia-Rong ^1,, ,
ZHANG Chi ^1,2 ,
FENG Tao ¹ ,
FU Li-Li ¹

1.
¹ China Jiliang University, Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province, National & Local United Engineering Lab of Quality Controlling Technology and Instrumentation for Marine Food, Hangzhou 310018, China;
2.
² Chongqing Academy of Metrology and Quality Inspection of Sixth Branch, Chongqing 408099, China

Corresponding author: PAN Jia-Rong,
Received Date: 3 May 2015
Available Online: 10 November 2015
Fund Project: 本文系国家质检公益专项(No.201210086)资助 (No.201210086)

A multi-residue analytical method was established for the simultaneous detection of ractopamine(RAC), clenbuterol(CL) and salbutamol(SAL) by suspension array technology. Based on the principle of indirect competitive enzyme linked immunosorbent assay, three veterinary antigens coupled with different fluorescent microspheres as probes were prepared and the phycoerythrin(PE)-labeled secondary antibodies were used as reporter signal. Standard curves of three veterinary drugs were separately established under optimum conditions. According to the specific recognition experiment, the standard curves for simultaneous detection of those three veterinary drugs were established. Good linearity was obtained in the concentration range of 1-500μg/L, 0.1-500μg/L, 1-100μg/L, and the detection limits were 0.68, 0.095 and 0.884μg/L for RAC, CL, SAL, respectively, with correlation coefficients(R²)>0.99 for all three drugs. The cross-reactivity of other structural analogues was lower than 1.5%. Good recoveries were found at four spiked levels for both single and mixed standards in real samples.
- Suspension array technology,
- Multi-residue analysis,
- Ractopamine,
- Clenbuterol,
- Salbutamol
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