Citation: Jichao XU, Ming HU, Xichang CHEN, Chunhui WANG, Leichen WANG, Lingyi ZHOU, Xing HE, Xiamin CHENG, Su JING. Construction and hydrogen peroxide-activated chemodynamic activity of ferrocene?benzoselenadiazole conjugate[J]. Chinese Journal of Inorganic Chemistry, ;2025, 41(8): 1495-1504. doi: 10.11862/CJIC.20250144 shu

Construction and hydrogen peroxide-activated chemodynamic activity of ferrocene?benzoselenadiazole conjugate

School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China

Corresponding author: Su JING, sjing@njtech.edu.cn
Received Date: 26 April 2025
Revised Date: 15 May 2025

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This article reports the rational design of a chemodynamic prodrug, FcNH-SeNBD. The prodrug was constructed by coupling ferrocene with benzoselenadiazole (SeNBD). By taking advantage of the elevated level of H₂O₂ in cancer cells, FcNH-SeNBD could generate cytotoxic ·OH radicals to induce cancer cell apoptosis and remain non-toxic in normal cells. In the meantime, the oxidation of ferrocene may interrupt its photo-induced electron transfer to SeNBD and facilitate the fluorescence recovery, thereby enabling FcNH-SeNBD for cancer cell imaging. FcNH-SeNBD was cytotoxic against liver cancer cell line HepG2 [IC₅₀=(7.95±0.98) μg·mL^-1] and colon cancer cell line HCT116 [IC₅₀=(15.74±1.5) μg·mL^-1], while displayed minimum toxicity to normal epithelial colonic cell line NCM- 460 (IC₅₀>100 μg·mL^-1), demonstrating its selective toxicity to cancer cell line. Mechanistic investigations revealed that FcNH-SeNBD was able to induce cancer cell apoptosis through caspase-3-dependent pathways. Moreover, a cell imaging study confirmed the strong red fluorescence signal of FcNH-SeNBD upon internalization and subsequent activation in tumor cells.
- ferrocene,
- benzoselenadiazole,
- prodrug,
- chemodynamic therapy,
- fluorescence imaging
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