Citation: Wei GAO, Meiqi SONG, Xuan REN, Jianliang BAI, Jing SU, Jianlong MA, Zhijun WANG. A self-calibrating fluorescent probe for the selective detection and bioimaging of HClO[J]. Chinese Journal of Inorganic Chemistry, ;2025, 41(6): 1173-1182. doi: 10.11862/CJIC.20250112 shu

A self-calibrating fluorescent probe for the selective detection and bioimaging of HClO

Department of Chemistry, Changzhi University, Changzhi, Shanxi 046011, China

Corresponding author: Jianlong MA, majianlong2021@163.com Zhijun WANG, wzhijun@sxu.edu.cn
Received Date: 2 April 2025
Revised Date: 9 May 2025

Figures(7)

In this study, a self-calibrating near-infrared fluorescence probe was designed and synthesized based on the dual-fluorophore strategy utilizing methylene blue and coumarin. The probe utilized methylene blue (emission spectrum range: 640-740 nm) and coumarin fluorophore (emission spectrum range: 440-600 nm) as signal output units, thereby achieving effective spectral separation and highly selective detection of HClO. Under physiological pH conditions, HClO triggers an oxidation-cleavage reaction, releasing methylene blue and coumarin, which emit distinct red and green fluorescence, respectively. This dualemission feature enabled rapid HClO detection with two-channel detection limits of 25.13 nmol·L^-1 (green channel) and 31.55 nmol·L^-1 (red channel). Furthermore, in cell imaging experiments, this probe demonstrated excellent cell membrane permeability and low cytotoxicity, successfully enabling the monitoring of both endogenous and exogenous HClO in living cells. By incorporating a two-channel self-calibration system, the probe effectively mitigated signal variations caused by instrumental or environmental interference, substantially improving detection sensitivity and reliability.
- fluorescent probe,
- fluorescent crosstalk,
- self-calibrated,
- hypochloric acid,
- dual channel
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