Citation: Yanxi LIU, Mengjia XU, Haonan CHEN, Quan LIU, Yuming ZHANG. A fluorescent-colorimetric probe for peroxynitrite-anion-imaging in living cells[J]. Chinese Journal of Inorganic Chemistry, ;2025, 41(6): 1112-1122. doi: 10.11862/CJIC.20240423 shu

A fluorescent-colorimetric probe for peroxynitrite-anion-imaging in living cells

Yanxi LIU ¹ ,
Mengjia XU ¹ ,
Haonan CHEN ¹ ,
Quan LIU ^{1, 2,,} ,
Yuming ZHANG ^1,,

1.
School of Chemistry and Chemical Engineering, Nantong University, Nantong, Jiangsu 226019, China
2.
Xinglin College, Nantong University, Nantong, Jiangsu 226008, China

Corresponding author: Quan LIU, liu.q@ntu.edu.cn Yuming ZHANG, zym81@ntu.edu.cn
Received Date: 2 December 2024
Revised Date: 20 March 2025

Figures(12)

Here, we report a near-infrared fluorescent probe, NSHD, which can be easily obtained through a one-step synthesis from a commercial dye. It quickly responded to peroxynitrite anion (ONOO^-) both in fluorescence and in colorimetric manners, with negligible interference from other common biological relative species or pH variations under physiological conditions. A good linear relationship was observed between emission intensity and concentration of ONOO^- in a range of 0-20 μmol·L^-1. The results of MS tests indicated that oxidative cleavages occurred upon ONOO^- exposure, resulting in changes to probe emission and color. NSHD successfully imaged the fluctuations of ONOO^- in living HeLa cells (human cervical cancer cells), treated by SIN-1 (3-morpholinosydnonimine) or co-incubated by LPS (lipopolysaccharide) and IFN-γ (interferon-γ).
- fluorescent probe,
- peroxynitrite anion,
- cell imaging
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