Citation: Linfang ZHANG, Wenzhu YIN, Gui YIN. A 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran-based near-infrared fluorescence probe for the detection of hydrogen sulfide and imaging of living cells[J]. Chinese Journal of Inorganic Chemistry, ;2025, 41(3): 540-548. doi: 10.11862/CJIC.20240405 shu

A 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran-based near-infrared fluorescence probe for the detection of hydrogen sulfide and imaging of living cells

Linfang ZHANG ¹ ,
Wenzhu YIN ² ,
Gui YIN ^{1, 3,,}

1.
School of Chemistry and Chemical Engineering, Yili Normal University, Yining, Xinjiang 835000, China
2.
Institute of Veterinary Immunology & Engineering, National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
3.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China

Corresponding author: Gui YIN, yingui@nju.edu.cn
Received Date: 12 November 2024
Revised Date: 16 December 2024

Figures(9)

Using 2-dicyanomethylene-3-cyano-4, 5, 5-trimethyl-2, 5-dihydrofuran (TCF) as a near-infrared fluorescent chromophore, we designed and synthesized a TCF-based fluorescent probe TCF-NS by introducing 2, 4-dinitrophenyl ether as the recognized site for H₂S. The probe TCF-NS displayed a rapid-response fluorescent against H₂S with high sensitivity and selection but had no significant fluorescence response to other biothiols. Furthermore, TCF-NS was applied to sense H₂S in living cells successfully with minimized cytotoxicity and a large Stokes shift.
- hydrogen sulfide,
- near-infrared fluorescence probe,
- cell imaging
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