Citation: Xiao-Yan CHENG, Meng XU, Jian-Ming OUYANG. Synthesis, Characterization, Adsorption Properties of Calcium Oxalate Monohydrate Crystals with Different Aspect Ratios and Their Toxicity to Renal Epithelial Cell[J]. Chinese Journal of Inorganic Chemistry, ;2022, 38(7): 1261-1271. doi: 10.11862/CJIC.2022.135 shu

Synthesis, Characterization, Adsorption Properties of Calcium Oxalate Monohydrate Crystals with Different Aspect Ratios and Their Toxicity to Renal Epithelial Cell

Institute of Biomineralization and Lithiasis Research, College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China

Corresponding author: Jian-Ming OUYANG, toyjm@jnu.edu.cn
Received Date: 7 December 2021
Revised Date: 20 April 2022

Figures(8)

Four kinds of calcium oxalate monohydrate (COM) crystals (COM-1:2, COM-1:3, COM-1:4, and COM-1:5) with the aspect ratio of 1:2, 1:3, 1:4, and 1:5, respectively, were synthesized. Their physicochemical properties were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscope (SEM), ζ potentiometer, and specific surface tester. SEM images show that the widths of the crystals were similar, but the lengths of the crystals were (3±0.3) μm, (5.2±0.3) μm, (7.0±0.7) μm, and (8.8±1.2) μm, respectively. With the increase of reaction temperature, the length of the crystal increased. The faster the stirring speed, the smaller the crystal size. As the concentration of the additive gelatin decreased, the (101) faces of COM crystals were elongated. Cell viability, cell total mortality, and reactive oxygen species (ROS) tests showed that the toxicity of COM with different length-width ratios to the human proximal tubular epithelial cells (HK-2) was COM-1:2 > COM-1:3 > COM-1:4 > COM-1:5 > control group. SEM examination showed that all four COM crystals could adhere to the cell surface. The reasons for this difference are positively related to the following points: a large proportion of (101) crystal planes, large specific surface area, and small cell-crystal shear stress.
- calcium oxalate monohydrate,
- size effect,
- cell damage,
- kidney stones,
- crystal plane
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