Citation: ZHANG Chang-Li, XU Jian, HUANG Fang, ZHAO Hai-Rong, CHEN Chang-Yun. A Highly Sensitive Ratiometric Fluorescence Probe for Zn²⁺ Detection in Living Cells[J]. Chinese Journal of Inorganic Chemistry, ;2020, 36(1): 97-105. doi: 10.11862/CJIC.2020.009 shu

A Highly Sensitive Ratiometric Fluorescence Probe for Zn²⁺ Detection in Living Cells

School of Environmental Science, Key Laboratory of Advanced Functional Materials of Nanjing, Nanjing Xiaozhuang University, Nanjing 211171, China

Corresponding author: ZHANG Chang-Li, carbon314@163.com
Received Date: 27 June 2019
Revised Date: 26 October 2019

Figures(6)

The emission spectra of 2-PBI (2-(2'-pyridyl) benzimidazole) show a red shift at a specific wavelength in both aqueous and acetonitrile solutions. However, 2-PBI cannot be used as ratiometric fluorescent probe for Zn²⁺ because of its low binding ability and variable coordination ratio to Zn²⁺. In this study, in order to add coordination atoms and promote the ICT effect of the probe by introducing N, N-dimethyl group at its 5-position of 2-PBI, design and synthesize a new ratiometric Zn²⁺ fluorescent probe DBITA. The results show that, besides large Stokes shift of 172 nm, DBITA exhibits the specific Zn²⁺-induced emission red-shift from 534 nm to 609 nm. DBITA combines with Zn²⁺ as 1:1 binding ratio. Moreover, DBITA displays the extremely high affinity toward Zn²⁺ of 0.16 pmol·L^-1. In HeLa cells, DBITA can be used to quantitatively detect intracellular Zn²⁺.
- zinc ions,
- fluorescence probe,
- ratiometric,
- quantitative,
- cell imaging
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

A Highly Sensitive Ratiometric Fluorescence Probe for Zn2+ Detection in Living Cells

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通讯作者: 陈斌, bchen63@163.com

A Highly Sensitive Ratiometric Fluorescence Probe for Zn²⁺ Detection in Living Cells