Citation: WEN Xiao-Ling, OUYANG Jian-Ming. Adsorption Properties of Bovine Serum Albumin on Micron/Nano Calcium Oxalate Monohydrate and Dihydrate Crystals[J]. Chinese Journal of Inorganic Chemistry, ;2016, 32(1): 103-110. doi: 10.11862/CJIC.2016.011 shu

Adsorption Properties of Bovine Serum Albumin on Micron/Nano Calcium Oxalate Monohydrate and Dihydrate Crystals

WEN Xiao-Ling ,
OUYANG Jian-Ming ^*,,

Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China

Corresponding author: OUYANG Jian-Ming, toyjm@jnu.edu.cn
Received Date: 10 August 2015
Revised Date: 27 September 2015

Figures(5)

The adsorption isotherms of bovine serum albumin (BSA) on calcium oxalate monohydrate (COM) and calcium oxalate dehydrate (COD) crystals with a size of 100 nm and 3 μm were detected, and Zeta potential of the crystals after adsorption of various concentration of BSA was measured. Adsorption quality change of BSA at different pH value were also measured. Results showed that the adsorption quality of BSA were positively related to the specific surface area of crystals, that was, following the order: COD-100nm > COM-100 nm≥COD-3 μm > COM-3 μm. However, after surface area normalization, the adsorption order was COD-3 μm > COM-3 μm > COM-100 nm > COD-100 nm. It indicated that the crystal water and charge density on crystal surface of COM and COD also affected BSA adsorption. These adsorption isotherms were fitted better with Langmuir model, indicating a monolayer adsorption of BSA on micron/nano COD and COM surface. The isoelectric point of the four crystals followed an order: COM-100 nm (7.2) > COD-100 nm (6.7)≥COM-3 μm (4.7) > COD-3 μm (4.3), which indicated more serious aggregation of nanocrystals under pathophysiological conditions (pH≈6.3). The maximum BSA adsorption occurred at around isoelectric point of BSA (pH=4.8). The adsorption of BSA on micron/nano COM is related to the specific surface area of crystals, its surface charge, crystal aggregation, crystal water number, and the pH value of system. These results can help to clarify the effect of various urine microcrystalline and urine proteins on the formation of CaO_x stones.
- nano,
- micron,
- calcium oxalate,
- bovine serum albumin,
- adsorption isotherms,
- isoelectric point,
- pH value
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