Citation: ZHANG Yong, ZHANG Zhe, DONG Xiong-Wei, YIN Wen-Xing, ZHANG Dan, LIU Chang-Lin. A Thioflavin T-Based Fluorescent Chelator Disaggregated Metal-Aβ Aggregates[J]. Chinese Journal of Inorganic Chemistry, ;2015, 31(8): 1495-1504. doi: 10.11862/CJIC.2015.233 shu

A Thioflavin T-Based Fluorescent Chelator Disaggregated Metal-Aβ Aggregates

1.
1. College of Chemistry, Central China Normal University, Wuhan 430079, China;
2.
2. College of Chemistry and Chemical Engineering, Hubei Polytechnic University, Huangshi, Hubei 435003, China

Corresponding author: LIU Chang-Lin,
Received Date: 31 October 2014
Available Online: 5 May 2015
Fund Project: 国家自然科学基金(No.21302059,21271079,21072074) (No.21302059,21271079,21072074)湖北省教育厅青年人才(No.Q20134402)资助项目。 (No.Q20134402)

A thioflavin T-based fluorescent chelator 5-amino-2-(benzo[d]thiazol-2-yl)phenol (FC-9) was synthesized and characterized. Its interaction with metal-Aβ aggregates and the change of morphology, conformation, cytotoxicity of the resulting Aβ aggregates were investigated. The results showed that FC-9 can not only recognize different Aβ40/Aβ42 aggregates but also disaggregate metal-Aβ40/Aβ42 aggregates. After the interaction between FC-9 and metal-Aβ40/Aβ42 aggregates, the morphology of aggregates changed from fibril to amorphousness, and their β-sheet conformation was also reduced. Moreover, the cell membrane penetration of FC-9 fulfilled common drug-like criteria, and it can inhibit cytotoxicity of the Cu-Aβ40 aggregates, indicating that it could have potential prospect in the treatment of Alzheimer disease. CCDC: 960540.
- Aβ aggregates;thioflavin T;fluorescent chelators;disaggregation
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