Citation: Yun-xiao Huang, Hu-sheng Yan. Effect of Buffer Types and Their Concentrations on ζ Potentials of Positively Charged Nanoparticles[J]. Acta Polymerica Sinica, ;2018, 0(7): 893-899. doi: 10.11777/j.issn1000-3304.2018.18007 shu

Effect of Buffer Types and Their Concentrations on ζ Potentials of Positively Charged Nanoparticles

Yun-xiao Huang ¹ ,
Hu-sheng Yan ^1,2,,

1.
Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071
2.
Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071

Corresponding author: Hu-sheng Yan, yanhs@nankai.edu.cn
Received Date: 8 January 2018
Revised Date: 29 March 2018
Available Online: 3 May 2018

The application of charged nanoparticles in drug delivery and imaging has been extensively investigated. The surface charge density of charged nanoparticles, which is usually characterized by ζ potentials, has a drastic effect on the interaction between the nanoparticles and the biological systems, and this interaction is critical for the in vivo biofate of the nanoparticles. However, the effect of different types of buffer systems and their concentrations on the ζ potentials is often ignored in literature. Various buffer systems, such as phosphate, Tris, Hepes and Mops, and different buffer concentrations from 1 mmol/L to 200 mmol/L, were used for measuring ζ potentials of nanoparticles. Herein the effect of buffer types and their concentrations on the ζ potentials for three different types of amine group-containing nanoparticles, i.e. poly(amido amine) (PAMAM) dendrimer, polystyrene-block-poly((N,N-diethylamino)ethyl methacrylate) (PS-b-PDMAEMA) micelles and chitosan nanoparticles, was studied. The ζ potentials of all the three types of nanoparticles decreased in the order of Tris, Hepes, Mops, and phosphates for buffer systems at the same concentration and pH. The ζ potentials were much lower in phosphate buffer than that in the others. The ζ potentials of amino group-containing chitosan nanoparticles showed even negative values in phosphate buffer with the concentration below ~ 10 mmol/L at pH = 7.4. The ζ potentials of all the three types of nanoparticles drastically decreased with the increase in buffer concentration (from 2 mmol/L to 100 mmol/L) for all the buffer systems investigated. The ζ potentials of chitosan nanoparticles in phosphate buffer (pH = 7.4) were reversed from positive to negative with the increase in phosphate concentration, with the crossover concentration of around 10 mmol/L. This charge reversal was contributed to the low protonation degree of chitosan amino groups (pK_a = 6.3) at pH = 7.4, and the presence of trivalent phosphate anions, which should be strongly adsorbed onto the positively charged particle surfaces and subsequently shielded the positive charges. When NaCl was added in the buffers, the ζ potentials of all the three types of nanoparticles decreased with increasing concentrations of the salt.
- Biomaterials,
- Drug delivery,
- ζ Potential,
- Positively charged nanoparticles
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