Citation: En-dong Zhang, Li-bing Liu, Feng-ting Lv, Shu Wang. Water-soluble Conjugated Polymers for Biosensor Applications[J]. Acta Polymerica Sinica, ;2018, (2): 186-197. doi: 10.11777/j.issn1000-3304.2018.17269 shu

Water-soluble Conjugated Polymers for Biosensor Applications

En-dong Zhang ^1,2 ,
Li-bing Liu ^1,2 ,
Feng-ting Lv ^1,2 ,
Shu Wang ^1,2,,

1.
Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190
2.
University of Chinese Academy of Sciences, Beijing 100049

Corresponding author: Shu Wang, wangshu@iccas.ac.cn
Received Date: 18 September 2017
Revised Date: 2 November 2017

In recent years, conjugated polymers (CPs) with characteristic and excellent photochemical and photophysical properties have become particularly popular in scientific research and are widely used for biological sensing. Conjugated polymers are characterized by a delocalized electronic structure along their backbones and exhibit electronic coupling between each optoelectronic segments. Owing to unique delocalized π-conjugated structure, CPs are endowed with distinguished optical properties, such as light-harvesting and amplification capability and high energy transfer efficiency. Water-soluble conjugated polymers (WSCPs) introduced with charged groups or selectively-recognition elements on the side chains can specifically diagnose through electrostatic interaction or specific binding with targets. In addition, WSCPs with diverse absorption and emission characteristics have been designed and synthesized by tuning the constitution and conformation of the backbones. In contrast with fluorescence proteins, small organic molecules and quantum dots, CPs with greatly unique properties have greater advantages that directed to sensing and imaging in biochemical and biomedical fields. This review article is aimed to summarize recent research progresses on applications of WSCPs as biosensors for the detection of a series of biological targets. Firstly, according to electrostatics interaction of CPs and targeted DNA, different novel strategies are used for detection of DNA mutations (section 2). Meanwhile, CPs modified with recognition moieties can be utilized to detect specific proteins (section 3). In addition, based on different interaction modes between CPs and cells and pathogens, identification and discrimination of microbial pathogens and cells are realized (section 4). CPs can also be used for cell imaging due to their advantageous optical properties (section 5). The structures, characteristics and advantages of CPs, as well as various nove lidentifications and detections strategies are also discussed. Through a variety of design and modification, novel CPs can be obtained and used for identification and detection, which is complementary to traditional CPs in biosensor and widens the range of biological applications. It is worth mentioning that, with the development of inter-discipline, more attention should be paid to the combination of new methods and strategies for exploiting functions of CPs in biological sensing.
- Conjugated polymer,
- Biosensor,
- Signal amplification,
- Identification and determination,
- Cell imaging
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