Citation: Luolin Wang, Xing Liang, Hanwen Chi, Weiying Lin. A dual-targeted near-infrared fluorescence lifetime probe for detecting viscosity heterogeneity in arthritic mice[J]. Chinese Chemical Letters, ;2025, 36(12): 110962. doi: 10.1016/j.cclet.2025.110962 shu

A dual-targeted near-infrared fluorescence lifetime probe for detecting viscosity heterogeneity in arthritic mice

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Institute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China

weiyinglin2013@163.com

Received Date: 28 September 2024
Revised Date: 12 February 2025
Accepted Date: 14 February 2025
Available Online: 15 February 2025

Figures(4)

Rheumatoid arthritis, being a chronic autoimmune malady, may culminate in joint malformation and incapacitation in severe instances. Nevertheless, monitoring the heterogeneity of the viscosity microenvironment in local joint areas remains challenging. Hence, we have developed a near-infrared (NIR)-emitting fluorescence lifetime probe WY-V for dual fluorescence lifetime imaging microscopy (FLIM)/NIR optical imaging, featuring precise targeting capabilities to the endoplasmic reticulum (ER) and lipid droplets (LD). This probe modulates distinct fluorescence lifetimes in the varying viscosity environments of these organelles, allowing for the quantification of their respective viscosities. Using FLIM/NIR optical imaging of joint tissues from arthritic mice, the probe accurately discerned the viscosity of inflamed cells at diverse sites, reveals the viscosity heterogeneity present within the arthritic tissues. Therefore, this research offers a potent biological instrument for clinical diagnosis and pathological examination of rheumatoid arthritis.
- Fluorescence lifetime imaging,
- NIR optical imaging,
- Arthritis,
- Heterogeneity,
- Endoplasmic reticulum,
- Lipid droplets
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