Citation: Jiaxi Wang, Zhiwei Gao, Hao Liang, Qianyue Liu, Weiqian Jin, Huyang Gao, Bailei Wang, Ruikai Zhu, Jiahao Huang, Xiaowen Li, Xingmou Wu, Weijiu Mo, Yinhan Liao, Ming Gao, Xiaojie Li, Cuiping Li. NIR stimulated epigallocatechin gallate loaded polydopamine with enhanced antibacterial and ROS scavenging abilities for improved infectious wound healing[J]. Chinese Chemical Letters, ;2025, 36(7): 110569. doi: 10.1016/j.cclet.2024.110569 shu

NIR stimulated epigallocatechin gallate loaded polydopamine with enhanced antibacterial and ROS scavenging abilities for improved infectious wound healing

Jiaxi Wang ^{a, 1} ,
Zhiwei Gao ^{b, 1} ,
Hao Liang ^{a, 1} ,
Qianyue Liu ^b ,
Weiqian Jin ^b ,
Huyang Gao ^b ,
Bailei Wang ^c ,
Ruikai Zhu ^d ,
Jiahao Huang ^b ,
Xiaowen Li ^a ,
Xingmou Wu ^a ,
Weijiu Mo ^a ,
Yinhan Liao ^a ,
Ming Gao ^{a, b, *,} ,
Xiaojie Li ^{a, *,} ,
Cuiping Li ^{a, e, *,}

a.
College & Hospital of Stomatology, Guangxi Medical University, Nanning 530021, China
b.
Life Sciences Institute, Guangxi Medical University, Nanning 530021, China
c.
Department of Critical Care Medicine, The Ninth Affiliated Hospital of Guangxi Medical University, Beihai 536000, China
d.
Department of Emergency, Guangxi Hospital Division of The First Affiliated Hospital, Sun Yat-sen University, Nanning 530022, China
e.
Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Medical University, Nanning 530021, China

gaoming1983125@hotmail.com

d52xiao@126.com

gxcuiping@sina.com

Received Date: 5 July 2024
Revised Date: 17 October 2024
Accepted Date: 21 October 2024
Available Online: 22 October 2024

Figures(3)

Infectious wound healing is complicated with and limited by infection and oxidative stress at the wound site. In recent years, various evidences suggest that nanozymes with multiple enzymatic activities have enabled the development of novel strategies for infectious wound healing. In this study, epigallocatechin gallate loaded polydopamine (P@E) was developed to act as a potent reactive oxygen species (ROS) scavenger for scavenging ROS, alleviating inflammatory responses, and promoting infectious wound healing. Combining with near infrared (NIR) irradiation, P@E presented excellent antibacterial ability of Escherichia coli (E. coli, 93.6%) and methicillin-resistant Staphylococcus aureus (MRSA, 87.6%). Specifically, P@E+NIR exhibited the most potent antioxidant, anti-inflammatory and cell proliferation behaviors through down-regulating intracellular ROS levels (81.9% and 94.3% for NIH3T3 and RAW264.7 respectively) and inducible nitric oxide synthase (iNOS) expression level (55.7%), and up-regulating the expression levels of arginase-1 (Arg-1, 71.4%), heat shock protein 70 (HSP70, 48.6%) and platelet endothelial cell adhesion molecule (CD31, 35.3%) compared to control group. Meanwhile, it also efficiently induced M2 directional polarization of lipopolysaccharide induced murine macrophages to achieve anti-inflammation, indicated by the down-regulation of CD86 (86.2%), and up-regulation of CD206 (85.6%). Significantly, it was also observed that P@E+NIR presented the excellent behaviors of inhibiting wound infection, alleviating wound inflammation, as well as promoting skin tissue repairing. Altogether, it has developed the strategy of using P@E combining with NIR irradiation for the synergistic enhanced healing of infectious skin wound, which can serve as a promising therapeutic strategy for its clinical treatment.
- Infectious wound healing,
- Nanozyme,
- Antibacterial activity,
- ROS scavenging,
- M2 directional polarization
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