Citation: Yiming Ma, Yuanbo Wang, Fang Wang, Sheng Lu, Xiaoqiang Chen. Site-specific protein labeling: Recent progress[J]. Chinese Chemical Letters, ;2025, 36(8): 110546. doi: 10.1016/j.cclet.2024.110546 shu

Site-specific protein labeling: Recent progress

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211800, China

shenglu@njtech.edu.cn

chenxq@njtech.edu.cn

Received Date: 25 July 2024
Revised Date: 9 October 2024
Accepted Date: 11 October 2024
Available Online: 15 October 2024

Figures(7)

Site-specific protein labeling plays important roles in drug discovery and illuminating biological processes at the molecular level. However, it is challenging to label proteins with high specificity while not affecting their structures and biochemical activities. Over the last few years, a variety of promising strategies have been devised that address these challenges including those that involve introduction of small-size peptide tags or unnatural amino acids (UAAs), chemical labeling of specific protein residues, and affinity-driven labeling. This review summarizes recent developments made in the area of site-specific protein labeling utilizing genetically encoding- and chemical-based methods, and discusses future issues that need to be addressed by researchers in this field.
- Protein labeling,
- Site specific labeling,
- Chemical labeling,
- Enzyme-mediated labeling,
- Peptide tags
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沈阳化工大学材料科学与工程学院沈阳 110142