Citation: Xiaolong Li, Changjiang Li, Chaopeng Shi, Jiarun Wang, Bei Yan, Xianjin Xiao, Tongbo Wu. CRISPR-Cas systems in DNA functional circuits: Strategies, challenges, prospects[J]. Chinese Chemical Letters, ;2025, 36(7): 110507. doi: 10.1016/j.cclet.2024.110507 shu

CRISPR-Cas systems in DNA functional circuits: Strategies, challenges, prospects

Xiaolong Li ^a ,
Changjiang Li ^a ,
Chaopeng Shi ^a ,
Jiarun Wang ^a ,
Bei Yan ^{b, *,} ,
Xianjin Xiao ^c ,
Tongbo Wu ^{a, *,}

a.
School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
b.
Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan 750004, China
c.
Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

D202081630@hust.edu.cn

wutongbo@hust.edu.cn

Received Date: 9 May 2024
Revised Date: 7 August 2024
Accepted Date: 24 September 2024
Available Online: 24 September 2024

Figures(5)

Strand displacement-based DNA circuits have emerged as highly effective tools for molecular computation, serving purposes of amplification or decision-making. They are favored for their inherent occurrence and sensitivity to external conditions. However, achieving enhanced amplification or decision-making necessitates the incorporation of multiple strands, thereby increasing the risk of contamination. Recent advancements have led to the development of CRISPR-Cas-based DNA circuits. These systems aim to simplify the complexity associated with conventional circuits, mitigate contamination risks, and enable more substantial amplification or decision-making capabilities. Here, the review article centers on current strategies of CRISPR-Cas (Cas9, Cas12a, Cas13a) system-assisted circuits in amplification and decision-making, and assesses their tendencies and limitations in amplification circuits and decision-making circuits. Furthermore, we discuss the challenges of CRISPR-Cas in circuits and propose prospects that will contribute to constructing more efficient and diverse CRISPR-Cas-based DNA functional circuits.
- CRISPR-Cas,
- DNA circuit,
- Amplification,
- Decision-making
1. [1]
  T.C. Xie, Y.H. Deng, J.R. Zhang, et al., Nucleic Acids Res. 50 (2022) 8431–8440. doi: 10.1093/nar/gkac650
2. [2]
  S. Okumura, G. Gines, N. Lobato-Dauzier, et al., Nature 610 (2022) 496–501. doi: 10.1038/s41586-022-05218-7
3. [3]
  M.P. Nikitin, Nat. Chem. 15 (2023) 70–82. doi: 10.1038/s41557-022-01111-y
4. [4]
  Y. Sun, S. Qi, X. Dong, et al., J. Hazard. Mater. 443 (2023) 130252.
5. [5]
  G. Shi, C. Yan, J. Chen, Anal. Chem. 93 (2021) 3273–3279. doi: 10.1021/acs.analchem.0c05173
6. [6]
  Q. Hu, H. Li, L. Wang, H. Gu, C. Fan, Chem. Rev. 119 (2019) 6459–6506. doi: 10.1021/acs.chemrev.7b00663
7. [7]
  S. Yang, J. Luo, L. Zhang, et al., Adv. Sci. 19 (2023) 2301814.
8. [8]
  J. Chen, S. Fu, C. Zhang, H. Liu, X. Su, Small 18 (2022) 2108008.
9. [9]
  C. Zhang, Y. Zhao, X. Xu, et al., Nat. Nanotechnol. 15 (2020) 709–715. doi: 10.1038/s41565-020-0699-0
10. [10]
  Q. Chao, Y. Zhang, Q. Li, et al., Anal. Chem. 95 (2023) 7723–7734. doi: 10.1021/acs.analchem.3c00898
11. [11]
  A.P. Lapteva, N. Sarraf, L. Qian, J. Am. Chem. Soc. 144 (2022) 12443–12449. doi: 10.1021/jacs.2c04325
12. [12]
  T. Wang, H. Hellmer, F.C. Simmel, Curr. Opin. Biotechnol. 79 (2023) 102867.
13. [13]
  D.Y. Zhang, E. Winfree, J. Am. Chem. Soc. 131 (2009) 17303–17314. doi: 10.1021/ja906987s
14. [14]
  C. Jung, A.D. Ellington, Acc. Chem. Res. 47 (2014) 1825–35. doi: 10.1021/ar500059c
15. [15]
  R.M. Dirks, N.A. Pierce, Proc. Natl. Acad. Sci. U.S.A. 101 (2004) 15275–15278.
16. [16]
  B.W. Zhang, T.R. Tian, D.X. Xiao, et al., Adv. Funct. Mater. 32 (2022) 2109728.
17. [17]
  Z.W. Luo, Y.X. Li, P. Zhang, et al., Trends Anal. Chem. 151 (2022) 116582.
18. [18]
  H.M. Wang, Y.Q. He, J. Wei, et al., Angew. Chem. Int. Ed. 61 (2022) 202115489.
19. [19]
  P. Yin, H.M. Choi, C.R. Calvert, N.A. Pierce, Nature 451 (2008) 318–22. doi: 10.1038/nature06451
20. [20]
  D.Y. Zhang, A.J. Turberfield, B. Yurke, E. Winfree, Science 318 (2007) 1148532.
21. [21]
  Q. Zhang, R. Zhao, C.C. Li, et al., Anal. Chem. 94 (2022) 13978–13986. doi: 10.1021/acs.analchem.2c03223
22. [22]
  S.Z. He, S.S. Yu, R.M. Li, et al., Angew. Chem. Int. Ed. 61 (2022) 202206529.
23. [23]
  R.M. Li, Y.X. Zhu, X. Gong, et al., J. Am. Chem. Soc. 145 (2023) 2999–3007. doi: 10.1021/jacs.2c11504
24. [24]
  J. Wei, M.D. Yu, K.Y. Tan, et al., Small 19 (2023) 2207961.
25. [25]
  P. Dong, R.M. Li, S.Z. He, et al., Chem. Sci. 14 (2023) 2159–2167. doi: 10.1039/d2sc05568f
26. [26]
  C. Zhang, L.L. Ge, Y.C. Zhuang, et al., Sci. China Inf. Sci. 62 (2019) 061301.
27. [27]
  F. Wang, H. Lv, Q. Li, et al., Nat. Commun. 11 (2020) 121.
28. [28]
  X. Song, J. Reif, ACS Nano 13 (2019) 6256–6268. doi: 10.1021/acsnano.9b02562
29. [29]
  J.K. Jung, C.M. Archuleta, K.K. Alam, J.B. Lucks, Nat. Chem. Biol. 18 (2022) 385–393. doi: 10.1038/s41589-021-00962-9
30. [30]
  S. Piranej, A. Bazrafshan, K. Salaita, Nat. Nanotechnol. 17 (2022) 514–523. doi: 10.1038/s41565-022-01080-w
31. [31]
  N. Xie, M.Q. Li, Y. Wang, et al., J. Am. Chem. Soc. 144 (2022) 9479–9488. doi: 10.1021/jacs.2c03258
32. [32]
  T. Song, A. Eshra, S. Shah, et al., Nat. Nanotechnol. 14 (2019) 1075–1081. doi: 10.1038/s41565-019-0544-5
33. [33]
  H. Hu, L.Q. Liu, L. Zhang, et al., Nano Res. 16 (2022) 865–872.
34. [34]
  K.M. Cherry, L. Qian, Nature 559 (2018) 370–376. doi: 10.1038/s41586-018-0289-6
35. [35]
  K. Shi, S.Y. Xie, R.Y. Tian, et al., Sci. Adv. 7 (2021) 7802.
36. [36]
  S.Y. Chen, B. Gong, C. Zhu, C.Y. Lei, Z. Nie, Trends Anal. Chem. 159 (2023) 116931.
37. [37]
  H.M. Li, Y. Xie, F.M. Chen, et al., Chem. Soc. Rev. 52 (2023) 361–382. doi: 10.1039/d2cs00594h
38. [38]
  W.R. Su, J.R. Li, C. Ji, et al., Nano Res. 16 (2023) 9940–9953. doi: 10.1007/s12274-023-5567-4
39. [39]
  Y. Li, S.Y. Li, J. Wang, G.Z. Liu, Trends Biotechnol. 37 (2019) 730–743. doi: 10.3390/catal9090730
40. [40]
  Z.Y. Weng, Z. You, J. Yang, et al., Angew. Chem. Int. Ed. 62 (2023) 202214987.
41. [41]
  R.J. Fu, Y.L. Xianyu, Small 19 (2023) 2300057.
42. [42]
  J.D. Habimana, R. Huang, B. Muhoza, et al., Biosens. Bioelectron. 203 (2022) 114033.
43. [43]
  X.K. Cheng, Y.R. Li, J. Kou, et al., Biosens. Bioelectron. 215 (2022) 114559.
44. [44]
  R. Montagud-Martinez, M. Heras-Hernandez, L. Goiriz, J.A. Daros, G. Rodrigo, A.C.S. Synth. Biol. 10 (2021) 950–956. doi: 10.1021/acssynbio.0c00649
45. [45]
  Y. Dai, R.A. Somoza, L. Wang, et al., Angew. Chem. Int. Ed. 58 (2019) 17399–17405. doi: 10.1002/anie.201910772
46. [46]
  H. Li, J. Yang, G. Wu, et al., Angew. Chem. Int. Ed. 61 (2022) 202203826.
47. [47]
  Z.Y. Li, X. Ding, K. Yin, et al., Biosens. Bioelectron. 192 (2021) 113498.
48. [48]
  D. Samanta, S.B. Ebrahimi, N. Ramani, C.A. Mirkin, J. Am. Chem. Soc. 144 (2022) 16310–16315. doi: 10.1021/jacs.2c07625
49. [49]
  C.M. Green, J. Spangler, K. Susumu, et al., ACS Nano 16 (2022) 20693–20704. doi: 10.1021/acsnano.2c07749
50. [50]
  W. Feng, A.M. Newbigging, J. Tao, et al., Chem. Sci. 12 (2021) 4683–4698. doi: 10.1039/d0sc06973f
51. [51]
  B.D. Huang, T.M. Groseclose, C.J. Wilson, Nat. Commun. 13 (2022) 3901.
52. [52]
  D.D. Zeng, J.L. Jiao, T.L. Mo, Front. Microbiol. 15 (2024) 1355234.
53. [53]
  J.L. Qiao, Z.Y. Zhao, Y.R. Li, et al., Crit. Rev. Food Sci. Nutr. (2023) 1–22. https://doi.org/10.1080/10408398.2023.2246558. doi: 10.1080/10408398.2023.2246558
54. [54]
  Y. Dhingra, S.K. Suresh, P. Juneja, D.G. Sashital, Mol. Cell 82 (2022) 4353–4367.
55. [55]
  L. Yu, M.A. Marchisio, Nucleic Acids Res. 51 (2023) 1473–1487. doi: 10.1093/nar/gkac1270
56. [56]
  J.S. Gootenberg, O.O. Abudayyeh, M.J. Kellner, et al., Science 360 (2018) 439–444. doi: 10.1126/science.aaq0179
57. [57]
  G. Petris, A. Casini, C. Montagna, et al., Nat. Commun. 8 (2017) 15334.
58. [58]
  J. Burian, V.K. Libis, Y.A. Hernandez, et al., Nat. Biotechnol. 41 (2023) 626–630. doi: 10.1038/s41587-022-01531-8
59. [59]
  J.C. Cofsky, K.M. Soczek, G.J. Knott, E. Nogales, J.A. Doudna, Nat. Struct. Mol. Biol. 29 (2022) 395–402. doi: 10.1038/s41594-022-00756-0
60. [60]
  T. Luo, J.C. Li, Y. He, et al., Anal. Chem. 94 (2022) 6566–6573. doi: 10.1021/acs.analchem.2c00401
61. [61]
  J.Y. Zhang, Z.Y. Li, C. Guo, et al., Angew. Chem. Int. Ed. (2024) 202403123.
62. [62]
  P. Jain, S. Rananaware, E. Vesco, et al., Nat. Commun. 14 (2023) 5409.
63. [63]
  S.Y. Li, Q.X. Cheng, J.K. Liu, et al., Cell Res. 28 (2018) 491–493. doi: 10.1038/s41422-018-0022-x
64. [64]
  J.S. Chen, E. Ma, L.B. Harrington, et al., Science 360 (2018) 436–439. doi: 10.1126/science.aar6245
65. [65]
  N. Mohammad, L. Talton, Z. Hetzler, M. Gongireddy, Q.S. Wei, Nucleic Acids Res. 51 (2023) 9894–9904. doi: 10.1093/nar/gkad715
66. [66]
  W. Zhang, Y.Q. Mu, K.J. Dong, et al., Nucleic Acids Res. 50 (2022) 12674–12688. doi: 10.1093/nar/gkac1144
67. [67]
  Z.J. Liu, J. Xu, S. Huang, et al., Biosens. Bioelectron. 247 (2024) 115936.
68. [68]
  Y. Wu, W. Luo, Z. Weng, et al., Nucleic Acids Res. 50 (2022) 11727–11737. doi: 10.1093/nar/gkac886
69. [69]
  O.O. Abudayyeh, J.S. Gootenberg, S. Konermann, et al., Science 353 (2016) 5573.
70. [70]
  N.K. Fan, X.T. Bian, M. Li, et al., Sci. Adv. 8 (2022) 7382.
71. [71]
  A. East-Seletsky, M.R. O'Connell, S.C. Knight, et al., Nature 538 (2016) 270–273. doi: 10.1038/nature19802
72. [72]
  K. Clement, J.Y. Hsu, M.C. Canver, J.K. Joung, L. Pinello, Mol. Cell 79 (2020) 11–29.
73. [73]
  H.R. Kempton, L.E. Goudy, K.S. Love, L.S. Qi, Mol. Cell 78 (2020) 184–191.
74. [74]
  S. Kawasaki, H. Ono, M. Hirosawa, et al., Nat. Commun. 14 (2023) 2243.
75. [75]
  L. Oesinghaus, F.C. Simmel, Nat. Commun. 10 (2019) 2092.
76. [76]
  W.J. Wang, Q.Y. Ge, X.W. Zhao, Trend. Anal. Chem. 160 (2023) 116960.
77. [77]
  I.M. Slaymaker, L.Y. Gao, B. Zetsche, et al., Science 351 (2016) 84–88. doi: 10.1126/science.aad5227
78. [78]
  Y. Liu, F. Pinto, X.Y. Wan, et al., Nat. Commun. 13 (2022) 1937. doi: 10.1109/icsp54964.2022.9778427
79. [79]
  M. Chen, D.M. Wu, S.H. Tu, et al., Biosens. Bioelectron. 173 (2020) 112821.
80. [80]
  J. Huang, Z. Liang, Y. Liu, J.D. Zhou, F.J. He, Anal. Chem. 94 (2022) 11409–11415. doi: 10.1021/acs.analchem.2c02538
81. [81]
  D.G. Zhang, L.J. Cai, X.W. Wei, et al., Nano Today 40 (2021) 101268.
82. [82]
  Y.H. Zhang, L. Qian, W.J. Wei, et al., ACS Synth. Biol. 6 (2017) 211–216. doi: 10.1021/acssynbio.6b00215
83. [83]
  B. Yurke, A.J. Turberfield, A.P. Mill, F.C. Simmel, J.L. Neumann, Nature 406 (2000) 605–608.
84. [84]
  X.B. Huang, Q. Zhou, M.X. Wang, et al., Front. Mol. Biosci. 7 (2020) 627848.
85. [85]
  Y.C. Gao, X. Xiong, S. Wong, et al., Nat. Methods 13 (2016) 1043. doi: 10.1038/nmeth.4042
86. [86]
  M. Bellato, A.F. Chiacchiera, E. Salibi, et al., Front. Bioeng. Biotechnol. 9 (2021) 743950.
87. [87]
  J. Santos-Moreno, Y. Schaerli, Biochem. Soc. Trans. 48 (2020) 1979–1993. doi: 10.1042/bst20200020
88. [88]
  A.K. Shaytan, R.V. Novikov, R.S. Vinnikov, A.K. Gribkova, G.S. Glukhov, Front. Mol. Biosci. 9 (2022) 1070526.
89. [89]
  H. Kim, D. Bojar, M. Fussenegger, Proc. Natl. Acad. Sci. U.S.A. 116 (2019) 7214–7219. doi: 10.1073/pnas.1821740116
90. [90]
  J. Elbaz, O. Lioubashevski, F. Wang, et al., Nat. Nanotechnol. 5 (2010) 417–422. doi: 10.1038/nnano.2010.88
91. [91]
  H. Pei, L. Liang, G.B. Yao, et al., Angew. Chem. Int. Ed. 51 (2012) 9020–9024. doi: 10.1002/anie.201202356
92. [92]
  D.C. Swarts, M. Jinek, Mol. Cell 73 (2019) 589–600.
93. [93]
  M. Rossetti, R. Merlo, N. Bagheri, et al., Nucleic Acids Res. 50 (2022) 8377–8391. doi: 10.1093/nar/gkac578
94. [94]
  S.D. Wang, H.J. Li, K.J. Dong, et al., Biosens. Bioelectron. 226 (2023) 115139.
95. [95]
  J. You, H. Park, H. Lee, et al., Biosens. Bioelectron. 224 (2023) 115078.
96. [96]
  M. Qing, S.L. Chen, Z. Sun, et al., Anal. Chem. 93 (2021) 7499–7507. doi: 10.1021/acs.analchem.1c00805
97. [97]
  H. Yan, Y.J. Wen, Z.M. Tian, et al., Nat. Biomed. Eng. 7 (2023) 1583–1601. doi: 10.1038/s41551-023-01033-1
98. [98]
  L. Liao, T.T. Gong, B.Y. Jiang, R. Yuan, Y. Xiang, Sensor. Actuator. B Chem. 379 (2023) 133221.
99. [99]
  S. Peng, Z. Tan, S.Y. Chen, C.Y. Lei, Z. Nie, Chem. Sci. 11 (2020) 7362–7368. doi: 10.1039/d0sc03084h
100. [100]
  Y. Lv, Y.H. Sun, Y. Zhou, et al., Small 19 (2023) 2206105.
101. [101]
  H.Y. Jia, H.L. Zhao, T. Wang, et al., Biosens. Bioelectron. 211 (2022) 114382.
102. [102]
  X.F. Wang, F.Y. Wang, J.R. Wang, et al., Sensor. Actuator. B: Chem. 370 (2022) 132480.
103. [103]
  C. Yan, G. Shi, J.H. Chen, J. Agric. Food Chem. 70 (2022) 12700–12707. doi: 10.1021/acs.jafc.2c04548
104. [104]
  S.H. Gong, X. Wang, P. Zhou, et al., Anal. Chem. 94 (2022) 15839–15846. doi: 10.1021/acs.analchem.2c03666
105. [105]
  J.F. Pan, F. Deng, Z. Liu, L.W. Zeng, J.H. Chen, Talanta 255 (2023) 124210.
106. [106]
  Z.F. Gao, L.L. Zheng, L.M. Dong, et al., Anal. Chem. 94 (2022) 6371–6379. doi: 10.1021/acs.analchem.2c00848
107. [107]
  X.M. Hang, P.F. Liu, S. Tian, et al., Biosens. Bioelectron. 211 (2022) 114393.
108. [108]
  C. Zhang, P.H. Zhang, H. Ren, et al., Chem. Eng. J. 446 (2022) 136864.
109. [109]
  J.J. Shen, X.M. Zhou, Y.Y. Shan, et al., Nat. Commun. 11 (2020) 267.
110. [110]
  Q. Han, X.L. Bian, Y. Chen, B.Z. Li, Sens. Actuators B: Chem. 393 (2023) 134265.
111. [111]
  Z.Y. Zhang, J.L. Li, C.L. Chen, et al., Anal. Chim. Acta 1300 (2024) 342409.
112. [112]
  J.J. Zhang, C.Y. Song, Y.F. Zhu, et al., Biosens. Bioelectron. 219 (2022) 114836.
113. [113]
  Y.H. Yang, J.C. Liu, X.H. Zhou, Biosens. Bioelectron. 190 (2021) 113418.
114. [114]
  Y.G. Ding, C. Tous, J. Choi, J. Chen, W.W. Wong, Nat. Commun. 15 (2024) 1572.
115. [115]
  T. Tian, B.W. Shu, Y.Z. Jiang, et al., ACS Nano 15 (2021) 1167–1178. doi: 10.1021/acsnano.0c08165
116. [116]
  Y. Sheng, T.H. Zhang, S.H. Zhang, et al., Biosens. Bioelectron. 178 (2021) 113027.
117. [117]
  P. Fozouni, S. Son, M.D.L. Derby, et al., Cell 184 (2021) 323–333.
118. [118]
  M.C. Zeng, Y.Q. Ke, Z.Y. Zhuang, et al., Anal. Chem. 94 (2022) 10805–10812. doi: 10.1021/acs.analchem.2c01588
119. [119]
  Y.B. Zhang, Y. Chen, Q.L. Zhang, Y.Z. Liu, X.J. Zhang, Biosens. Bioelectron. 230 (2023) 115248.
120. [120]
  S. Nimkar, B. Anand, Nucleic Acids Res. 48 (2020) 2486–2501. doi: 10.1093/nar/gkz1218
121. [121]
  C.Y. Hu, D.C. Ni, K.H. Nam, et al., Mol. Cell 82 (2022) 2754–2768. doi: 10.3390/plants11202754
122. [122]
  K.E. Dillard, M.W. Brown, N.V. Johnson, et al., Cell 175 (2018) 934–946.
123. [123]
  K. Yoshimi, K. Takeshita, N. Kodera, et al., Nat. Commun. 13 (2022) 4917.
124. [124]
  Y.B. Xiao, M. Luo, A.E. Dolan, M.F. Liao, A. Ke, Science 361 (2018) eaat0839.
125. [125]
  Y.W. Huo, K.H. Nam, F. Ding, et al., Nat. Struct. Mol. Biol. 21 (2014) 771–777. doi: 10.1038/nsmb.2875
126. [126]
  H. Morisaka, K. Yoshimi, Y. Okuzaki, et al., Nat. Commun. 10 (2019) 5302.
127. [127]
  R.J. Xiao, Z. Li, S.K. Wang, R.J. Han, L.F. Chang, Nucleic Acids Res. 49 (2021) 4120–4128. doi: 10.1093/nar/gkab179
128. [128]
  L.B. Harrington, D. Burstein, J.S. Chen, et al., Science 362 (2018) 839–842. doi: 10.1126/science.aav4294
129. [129]
  T. Karvelis, G. Bigelyte, J.K. Young, et al., Nucleic Acids Res. 48 (2020) 5016–5023. doi: 10.1093/nar/gkaa208
130. [130]
  X.S. Xu, A. Chemparathy, L.P. Zeng, et al., Mol. Cell 81 (2021) 4333–4345.
131. [131]
  P. Wu, X.S. Ye, D.Q. Wang, et al., J. Hazard. Mater. 424 (2022) 127690.
132. [132]
  M.M. Chen, J.Y. Zhang, Y. Peng, et al., Biosens. Bioelectron. 218 (2022) 114792.
133. [133]
  B. Csörgő, L.M. León, I.J. Chau-Ly, et al., Nat. Methods 17 (2020) 1183–1190. doi: 10.1038/s41592-020-00980-w
134. [134]
  C. Kuscu, S. Arslan, R. Singh, J. Thorpe, M. Adli, Nat. Biotechnol. 32 (2014) 677–683. doi: 10.1038/nbt.2916
135. [135]
  H. Mangkhwar, B. Li, X. Ding, et al., Adv. Sci. 7 (2020) 1902312.
136. [136]
  H.Y. Xiao, J.Y. Hu, C. Huang, et al., Trends Anal. Chem. 161 (2023) 117000.
137. [137]
  M. Karlikow, E. Amalfitano, X. Yang, et al., Nat. Commun. 14 (2023) 1505.
138. [138]
  F. Deng, Y. Li, B.Y. Yang, et al., Nat. Commun. 15 (2024) 1818.
139. [139]
  K. Sun, L. Pu, C. Chen, et al., Nucleic Acids Res. 52 (2024) 1–13.
1. [1]
  Kuangdi Luo , Yang Qin , Xuehao Zhang , Hanxu Ji , Heao Zhang , Jiangtian Li , Xianjin Xiao , Xinyu Wang . Regulable toehold lock for the effective control of strand displacement reaction sequence and circuit leakage. Chinese Chemical Letters, 2024, 35(7): 109104-. doi: 10.1016/j.cclet.2023.109104
2. [2]
  Yang Qin , Jiangtian Li , Xuehao Zhang , Kaixuan Wan , Heao Zhang , Feiyang Huang , Limei Wang , Hongxun Wang , Longjie Li , Xianjin Xiao . Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex. Chinese Chemical Letters, 2024, 35(5): 108826-. doi: 10.1016/j.cclet.2023.108826
3. [3]
  Minghao Hu , Tianci Xie , Yuqiang Hu , Longjie Li , Ting Wang , Tongbo Wu . Allosteric DNAzyme-based encoder for molecular information transfer. Chinese Chemical Letters, 2024, 35(7): 109232-. doi: 10.1016/j.cclet.2023.109232
4. [4]
  Yuqing Liu , Shiling Zhang , Kai Jiang , Shiyue Ding , Limei Xu , Yingqi Liu , Ting Wang , Fenfen Zheng , Weiwei Xiong , Jun-Jie Zhu . Near-infrared light responsive upconversion-DNA nanocapsules for remote-controlled CRISPR-Cas9 genome editing. Chinese Chemical Letters, 2025, 36(5): 110282-. doi: 10.1016/j.cclet.2024.110282
5. [5]
  Yan Liu , Yang Wang , Jiayi Zhu , Xuxian Su , Xudong Lin , Liang Xu , Xiwen Xing . Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells. Chinese Chemical Letters, 2024, 35(9): 109427-. doi: 10.1016/j.cclet.2023.109427
6. [6]
  Haijiang Gong , Qingtan Zeng , Shili Gai , Yaqian Du , Jing Zhang , Qingyu Wang , He Ding , Lichun Wu , Anees Ahmad Ansari , Piaoping Yang . Enzyme-based colorimetric signal amplification strategy in lateral flow immunoassay. Chinese Chemical Letters, 2025, 36(5): 110059-. doi: 10.1016/j.cclet.2024.110059
7. [7]
  Ruixin Liu , Feng Shi , Yanping Xia , Haibing Zhu , Jiawen Cao , Kai Peng , Chuanli Ren , Juan Li , Zhanjun Yang . Universal MOF nanozyme-induced catalytic amplification strategy for label-free electrochemical immunoassay. Chinese Chemical Letters, 2024, 35(11): 109664-. doi: 10.1016/j.cclet.2024.109664
8. [8]
  Qinghong Pan , Huafang Zhang , Qiaoling Liu , Donghong Huang , Da-Peng Yang , Tianjia Jiang , Shuyang Sun , Xiangrong Chen . A self-powered cathodic molecular imprinting ultrasensitive photoelectrochemical tetracycline sensor via ZnO/C photoanode signal amplification. Chinese Chemical Letters, 2025, 36(1): 110169-. doi: 10.1016/j.cclet.2024.110169
9. [9]
  Bo Liu , Shuaiqiang Shao , Junjie Cai , Zijian Zhang , Feng Tian , Kun Yang , Fan Li . Signal cascade amplification of streptavidin-biotin-modified immunofluorescence nanocapsules for ultrasensitive detection of glial fibrillary acidic protein. Chinese Chemical Letters, 2025, 36(3): 109814-. doi: 10.1016/j.cclet.2024.109814
10. [10]
  Xueying Shi , Xiaoxuan Zhou , Bing Xiao , Hongxia Xu , Wei Zhang , Hongjie Hu , Shiqun Shao , Zhuxian Zhou , Youqing Shen , Xiaodan Xu , Jianbin Tang . A β-lapachone-loaded iron-polyphenol nanocomplex enhances chemodynamic therapy through cascade amplification of ROS in tumor. Chinese Chemical Letters, 2025, 36(5): 110178-. doi: 10.1016/j.cclet.2024.110178
11. [11]
  Feng Shi , Guiling Li , Haibing Zhu , Ling Li , Ming Chen , Juan Li , Huifang Shen , Hao Zeng , Lingfeng Min , Zhanjun Yang . Nanozyme-triggered polymerization amplification strategy for constructing highly sensitive surface plasmon resonance immunosensing. Chinese Chemical Letters, 2025, 36(6): 110333-. doi: 10.1016/j.cclet.2024.110333
12. [12]
  Shuo Tian , Shuyun Chen , Yunsen Wang , Dianping Tang . Liposomal photoelectrochemical immunoassay for low-abundance proteins with ternary transition metal sulfides for signal amplification. Chinese Chemical Letters, 2025, 36(7): 110418-. doi: 10.1016/j.cclet.2024.110418
13. [13]
  Yanqi Wu , Yuhong Guan , Peilin Huang , Hui Chen , Liping Bai , Zhihong Jiang . Preparation of norovirus GII loop mediated isothermal amplification freeze-drying microsphere reagents and its application in an on-site integrated rapid detection platform. Chinese Chemical Letters, 2024, 35(9): 109308-. doi: 10.1016/j.cclet.2023.109308
14. [14]
  Sixin Ai , Wenxiu Li , Huayong Zhu , Yang Wan , Weiying Lin . Viscosity-responsive signal amplification dual-modal probe triggered by cysteine/homocysteine for monitoring diabetic liver damages and repair processes. Chinese Chemical Letters, 2025, 36(3): 109904-. doi: 10.1016/j.cclet.2024.109904
15. [15]
  Chang Liu , Tao Wu , Lijiao Deng , Xuzi Li , Xin Fu , Shuzhen Liao , Wenjie Ma , Guoqiang Zou , Hai Yang . Programmed DNA walkers for biosensors. Chinese Chemical Letters, 2024, 35(9): 109307-. doi: 10.1016/j.cclet.2023.109307
16. [16]
  Jia-Li Xie , Tian-Jin Xie , Yu-Jie Luo , Kai Mao , Cheng-Zhi Huang , Yuan-Fang Li , Shu-Jun Zhen . Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection. Chinese Chemical Letters, 2024, 35(6): 109137-. doi: 10.1016/j.cclet.2023.109137
17. [17]
  Xiaohong Wen , Mei Yang , Lie Li , Mingmin Huang , Wei Cui , Suping Li , Haiyan Chen , Chen Li , Qiuping Guo . Enzymatically controlled DNA tetrahedron nanoprobes for specific imaging of ATP in tumor. Chinese Chemical Letters, 2024, 35(8): 109291-. doi: 10.1016/j.cclet.2023.109291
18. [18]
  Jingwen Zhao , Jianpu Tang , Zhen Cui , Limin Liu , Dayong Yang , Chi Yao . A DNA micro-complex containing polyaptamer for exosome separation and wound healing. Chinese Chemical Letters, 2024, 35(9): 109303-. doi: 10.1016/j.cclet.2023.109303
19. [19]
  Zhongyu Wang , Lijun Wang , Huaixin Zhao . DNA-based nanosystems to generate reactive oxygen species for nanomedicine. Chinese Chemical Letters, 2024, 35(11): 109637-. doi: 10.1016/j.cclet.2024.109637
20. [20]
  Jiangshan Xu , Weifei Zhang , Zhengwen Cai , Yong Li , Long Bai , Shaojingya Gao , Qiang Sun , Yunfeng Lin . Tetrahedron DNA nanostructure/iron-based nanomaterials for combined tumor therapy. Chinese Chemical Letters, 2024, 35(11): 109620-. doi: 10.1016/j.cclet.2024.109620

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(5)
HTML views(1)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142