Citation: Fangbing Wang, Qiankun Zeng, Jing Ren, Min Zhang, Guoyue Shi. A membrane-based plasma separator coupled with ratiometric fluorescent sensor for biochemical analysis in whole blood[J]. Chinese Chemical Letters, ;2025, 36(7): 110494. doi: 10.1016/j.cclet.2024.110494 shu

A membrane-based plasma separator coupled with ratiometric fluorescent sensor for biochemical analysis in whole blood

School of Chemistry and Molecular Engineering, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, Shanghai 200241, China

Corresponding author: Min Zhang, mzhang@chem.ecnu.edu.cn Guoyue Shi, gyshi@chem.ecnu.edu.cn
Received Date: 2 November 2023
Revised Date: 28 August 2024
Accepted Date: 23 September 2024
Available Online: 24 September 2024

Figures(9)

Herein, we reported an integrated device that was utilized to directly separate plasma and analyze glucose (Glu), cholesterol (Chol) from whole blood samples. The separating module primarily consists of a porous asymmetric polysulfone membrane. The vertical placement of membrane and the gravity settlement of blood cells can reduce mechanical damage to blood cells and blockage of the membrane, resulting in improved separation efficiency of the membrane. The detection module consists of a smart phone and a ratio fluorescence sensing system based on NH₂−MIL-53(Al) and o-phenylenediamine (OPD). The sensing system presents a dual emission response to H₂O₂ the main oxidation product of Glu and Chol. Due to the fluorescence resonance energy transfer (FRET), the response of the fluorescence intensity ratio (F_{574 nm}/F_{434 nm} or F_{554 nm}/F_{434 nm}) gradually increases with increasing H₂O₂ concentration, accompanied by a color change from weak to strong. The visual detection of Glu and Chol can be realized through the recognition of RGB values by smart phones. The integrated device has been successfully used to analysis Glu and Chol in real blood samples, which provided a universal platform for sensing biocatalytic processes with H₂O₂ production.
- NH₂-MIL-53(Al),
- Cholesterol,
- Glucose,
- Colorimetric,
- Blood plasma separation
1. [1]
  C.O. Adetunji, O.S. Michael, S. Rathee, et al., Mater. Today Adv. 13 (2021) 100198.
2. [2]
  S. O'Neill, L. O'Driscoll, Obes. Rev. 16 (2015) 1–12. doi: 10.1111/obr.12229
3. [3]
  J. Khunger, M. Malhotra, N. Kumar, et al., Blood 134 (2019) 4959. doi: 10.1182/blood-2019-125048
4. [4]
  J. Gall, E. Frisdal, R. Bittar, et al., Atherosclerosis 252 (2016) e205.
5. [5]
  H. Takase, K. Hayashi, F. Kin, et al., Hypertens. Res. 46 (2023) 236–243. doi: 10.1038/s41440-022-01035-7
6. [6]
  L. Haverals, K.V. Dessel, A. Verrijken, et al., Nutr. Diabetes 9 (2019) 16.
7. [7]
  D.P. Yang, W.W. Guo, Z.F. Cai, et al., Sens. Actuators B: Chem. 260 (2018) 642–649.
8. [8]
  J. Gao, W.Z. Huang, Z.P. Chen, C.Q. Yi, L.L. Jiang, Sens. Actuators B: Chem. 287 (2019) 102–110.
9. [9]
  C.B. Ma, Y. Zhang, Q. Liu, Y. Du, E.K. Wang, Anal. Chem. 92 (2020) 5319–5328. doi: 10.1021/acs.analchem.9b05858
10. [10]
  C.Y. Hong, X.X. Zhang, C.Y. Wu, et al., ACS Appl. Mater. Interfaces 12 (2020) 54426–54432. doi: 10.1021/acsami.0c15900
11. [11]
  S. Singh, M. Singh, K. Mitra, et al., Electrochim. Acta 258 (2017) 1435–1444.
12. [12]
  Z.L. Yuan, S.H. Chen, B. Liu, J. Mater. Sci. 52 (2017) 164–172. doi: 10.1007/s10853-016-0318-5
13. [13]
  Z.J. Liu, L. Wang, P.F. Liu, et al., Food Chem. 357 (2021) 129753.
14. [14]
  W.X. Lv, X. Wan, J.H. Wu, et al., Chin. Chem. Lett. 30 (2019) 1635–1638.
15. [15]
  R.G. Gui, H. Jin, X.N. Bu, et al., Coord. Chem. Rev. 383 (2019) 82–103.
16. [16]
  L. Guo, Y. Liu, R.M. Kong, et al., Anal. Chem. 91 (2019) 2453–12460.
17. [17]
  M. Kersaudy-Kerhoas, E. Sollier, Lab Chip 13 (2013) 3323–3346. doi: 10.1039/c3lc50432h
18. [18]
  B. Kim, S. Oh, D.W. You, S.Y. Choi, Anal. Chem. 89 (2017) 1439–1444. doi: 10.1021/acs.analchem.6b04587
19. [19]
  M.F. Ulum, L. Maylina, D. Noviana, D.H.B. Wicaksono, Lab Chip 16 (2016) 1492–1504.
20. [20]
  C.C. Liu, S.C. Liao, J.Z. Song, et al., Lab Chip 16 (2016) 553–560.
21. [21]
  S.S. Zhou, T. Hu, F. Zhang, et al., Anal. Chem. 92 (2020) 1574–1581. doi: 10.1021/acs.analchem.9b04852
22. [22]
  A. Tajudin, K. Petersson, A. Lenshof, et al., Lab Chip 13 (2013) 1790–1796. doi: 10.1039/c3lc41269e
23. [23]
  X.B. Zhang, Z.Q. Wu, K. Wang, et al., Anal. Chem. 84 (2012) 3780–3786. doi: 10.1021/ac3003616
24. [24]
  J. Hauser, G. Lenk, J. Hansson, et al., Anal. Chem. 90 (2018) 13393–13399. doi: 10.1021/acs.analchem.8b03175
25. [25]
  J. Hauser, G. Lenk, S. Ullah, et al., Anal. Chem. 91 (2019) 7125–7130. doi: 10.1021/acs.analchem.9b00204
26. [26]
  W.S. Mielczarek, E.A. Obaje, T.T. Bachmann, M. Kersaudy-Kerhoas, Lab Chip 16 (2016) 3441–3448.
27. [27]
  C.C. Lien, P.J. Chen, A. Venault, et al., J. Membr. Sci. 584 (2019) 148–160.
28. [28]
  H.N. Zhao, Z.P. Xing, S.Y. Su, et al., Appl. Catal. B: Environ. 291 (2021) 120106.
29. [29]
  K.R. Baillargeon, L.P. Murray, R.N. Deraney, C.R. Mace, Anal. Chem. 92 (2020) 16245–16252. doi: 10.1021/acs.analchem.0c04127
30. [30]
  J.H. Son, S.H. Lee, S. Hong, et al., Lab Chip 14 (2014) 2287–2292.
31. [31]
  Y.B. Guan, M., Xia, X.H. Wang, W.C. Cao, A. Marchettib, Inorganica Chim Acta 484 (2019) 180–184.
32. [32]
  Y.H. Yuan, J.Z. Jiang, S.P. Liu, et al., Sens. Actuators B: Chem. 242 (2017) 545–553.
33. [33]
  Y. Zhao, H. Liu, Y.N. Jiang, et al., Anal. Chem. 90 (2018) 14578–14585. doi: 10.1021/acs.analchem.8b04691
34. [34]
  J.L. Ding, W.Q. Zhang, F.Y. Xue, et al., Microchim. Acta 189 (2022) 203.
35. [35]
  H.V. Xu, Y. Zhao, Y.N. Tan, ACS Appl. Mater. Interfaces 11 (2019) 27233–27242. doi: 10.1021/acsami.9b08708
36. [36]
  F. Qu, Q.Q. Yang, B.J. Wang, J.M. You, Talanta 207 (2020) 120289.
37. [37]
  S.S. Jiang, Y.F. Zhang, Y.C. Yang, et al., ACS Appl. Mater. Interfaces 11 (2019) 10554–10558. doi: 10.1021/acsami.9b00010
38. [38]
  W.R. Zhang, Y.M. Zhang, F.L. Xie, et al., Adv. Mater. 32 (2020) 2003121.
39. [39]
  K.K. Zheng, Y. Tong, S.H. Zhang, et al., Adv. Funct. Mater. 31 (2021) 2102599.
40. [40]
  X.Y. Lu, X.J. Shi, A. Hu, et al., Nature 588 (2020) 479–484. doi: 10.1038/s41586-020-2928-y
1. [1]
  Erzhuo Cheng , Yunyi Li , Wei Yuan , Wei Gong , Yanjun Cai , Yuan Gu , Yong Jiang , Yu Chen , Jingxi Zhang , Guangquan Mo , Bin Yang . Galvanostatic method assembled ZIFs nanostructure as novel nanozyme for the glucose oxidation and biosensing. Chinese Chemical Letters, 2024, 35(9): 109386-. doi: 10.1016/j.cclet.2023.109386
2. [2]
  Jia Chen , Yun Liu , Zerong Long , Yan Li , Hongdeng Qiu . Colorimetric detection of α-glucosidase activity using Ni-CeO₂ nanorods and its application to potential natural inhibitor screening. Chinese Chemical Letters, 2024, 35(9): 109463-. doi: 10.1016/j.cclet.2023.109463
3. [3]
  Gengchen Guo , Tianyu Zhao , Ruichang Sun , Mingzhe Song , Hongyu Liu , Sen Wang , Jingwen Li , Jingbin Zeng . Au-Fe₃O₄ dumbbell-like nanoparticles based lateral flow immunoassay for colorimetric and photothermal dual-mode detection of SARS-CoV-2 spike protein. Chinese Chemical Letters, 2024, 35(6): 109198-. doi: 10.1016/j.cclet.2023.109198
4. [4]
  Haijiang Gong , Qingtan Zeng , Shili Gai , Yaqian Du , Jing Zhang , Qingyu Wang , He Ding , Lichun Wu , Anees Ahmad Ansari , Piaoping Yang . Enzyme-based colorimetric signal amplification strategy in lateral flow immunoassay. Chinese Chemical Letters, 2025, 36(5): 110059-. doi: 10.1016/j.cclet.2024.110059
5. [5]
  Jiahui Li , Qiao Shi , Ying Xue , Mingde Zheng , Long Liu , Tuoyu Geng , Daoqing Gong , Minmeng Zhao . The effects of in ovo feeding of selenized glucose on liver selenium concentration and antioxidant capacity in neonatal broilers. Chinese Chemical Letters, 2024, 35(6): 109239-. doi: 10.1016/j.cclet.2023.109239
6. [6]
  Yue Sun , Yingnan Zhu , Jiahang Si , Ruikang Zhang , Yalan Ji , Jinjie Fan , Yuze Dong . Glucose-activated nanozyme hydrogels for microenvironment modulation via cascade reaction in diabetic wound. Chinese Chemical Letters, 2025, 36(4): 110012-. doi: 10.1016/j.cclet.2024.110012
7. [7]
  Guorong Li , Yijing Wu , Chao Zhong , Yixin Yang , Zian Lin . Predesigned covalent organic framework with sulfur coordination: Anchoring Au nanoparticles for sensitive colorimetric detection of Hg(Ⅱ). Chinese Chemical Letters, 2024, 35(5): 108904-. doi: 10.1016/j.cclet.2023.108904
8. [8]
  Caixia Zhu , Qing Hong , Kaiyuan Wang , Yanfei Shen , Songqin Liu , Yuanjian Zhang . Single nanozyme-based colorimetric biosensor for dopamine with enhanced selectivity via reactivity of oxidation intermediates. Chinese Chemical Letters, 2024, 35(10): 109560-. doi: 10.1016/j.cclet.2024.109560
9. [9]
  Kezuo Di , Jie Wei , Lijun Ding , Zhiying Shao , Junling Sha , Xilong Zhou , Huadong Heng , Xujing Feng , Kun Wang . A wearable sensor device based on screen-printed chip with biofuel cell-driven electrochromic display for noninvasive monitoring of glucose concentration. Chinese Chemical Letters, 2025, 36(2): 109911-. doi: 10.1016/j.cclet.2024.109911
10. [10]
  Yuxin Wang , Zhengxuan Song , Yutao Liu , Yang Chen , Jinping Li , Libo Li , Jia Yao . Methyl functionalization of trimesic acid in copper-based metal-organic framework for ammonia colorimetric sensing at high relative humidity. Chinese Chemical Letters, 2024, 35(6): 108779-. doi: 10.1016/j.cclet.2023.108779
11. [11]
  Maosen Xu , Pengfei Zhu , Qinghong Cai , Meichun Bu , Chenghua Zhang , Hong Wu , Youzhou He , Min Fu , Siqi Li , Xingyan Liu . In-situ fabrication of TiO₂/NH₂−MIL-125(Ti) via MOF-driven strategy to promote efficient interfacial effects for enhancing photocatalytic NO removal activity. Chinese Chemical Letters, 2024, 35(10): 109524-. doi: 10.1016/j.cclet.2024.109524
12. [12]
  Yongheng Ren , Yang Chen , Hongwei Chen , Lu Zhang , Jiangfeng Yang , Qi Shi , Lin-Bing Sun , Jinping Li , Libo Li . Electrostatically driven kinetic Inverse CO2/C2H2 separation in LTA-type zeolites. Chinese Journal of Structural Chemistry, 2024, 43(10): 100394-100394. doi: 10.1016/j.cjsc.2024.100394
13. [13]
  Huirong Chen , Yingzhi He , Yan Han , Jianbo Hu , Jiantang Li , Yunjia Jiang , Basem Keshta , Lingyao Wang , Yuanbin Zhang . A new SIFSIX anion pillared cage MOF with crs topological structure for efficient C2H2/CO2 separation. Chinese Journal of Structural Chemistry, 2025, 44(2): 100508-100508. doi: 10.1016/j.cjsc.2024.100508
14. [14]
  Wenbi Wu , Yinchu Dong , Haofan Liu , Xuebing Jiang , Li Li , Yi Zhang , Maling Gou . Modification of plasma protein for bioprinting via photopolymerization. Chinese Chemical Letters, 2024, 35(8): 109260-. doi: 10.1016/j.cclet.2023.109260
15. [15]
  Zhen Shi , Wei Jin , Yuhang Sun , Xu Li , Liang Mao , Xiaoyan Cai , Zaizhu Lou . Interface charge separation in Cu2CoSnS4/ZnIn2S4 heterojunction for boosting photocatalytic hydrogen production. Chinese Journal of Structural Chemistry, 2023, 42(12): 100201-100201. doi: 10.1016/j.cjsc.2023.100201
16. [16]
  Xiaxi Yao , Xiuli Hu , Fangcheng Huang , Xuhong Wang , Xuekun Hong , Dawei Wang . Improved hydrogen and oxygen evolution rates in Pt@TiO₂@RuO₂ hollow nanoshells through dielectric Mie resonance and spatial cocatalyst separation. Chinese Chemical Letters, 2025, 36(5): 110192-. doi: 10.1016/j.cclet.2024.110192
17. [17]
  Yanhua Chen , Xian Ding , Jun Zhou , Zhaoying Wang , Yunhai Bo , Ying Hu , Qingce Zang , Jing Xu , Ruiping Zhang , Jiuming He , Fen Yang , Zeper Abliz . Plasma metabolomics combined with mass spectrometry imaging reveals crosstalk between tumor and plasma in gastric cancer genesis and metastasis. Chinese Chemical Letters, 2025, 36(1): 110351-. doi: 10.1016/j.cclet.2024.110351
18. [18]
  Han Wu , Yumei Wang , Zekai Ren , Hailin Cong , Youqing Shen , Bing Yu . The nanocarrier strategy for crossing the blood-brain barrier in glioma therapy. Chinese Chemical Letters, 2025, 36(4): 109996-. doi: 10.1016/j.cclet.2024.109996
19. [19]
  Fahui Xiang , Lu Li , Zhen Yuan , Wuji Wei , Xiaoqing Zheng , Shimin Chen , Yisi Yang , Liangji Chen , Zizhu Yao , Jianwei Fu , Zhangjing Zhang , Shengchang Xiang . Enhanced C₂H₂/CO₂ separation in tetranuclear Cu(Ⅱ) cluster-based metal-organic frameworks by adjusting divider length of pore space partition. Chinese Chemical Letters, 2025, 36(3): 109672-. doi: 10.1016/j.cclet.2024.109672
20. [20]
  Ningning Gao , Yue Zhang , Zhenhao Yang , Lijing Xu , Kongyin Zhao , Qingping Xin , Junkui Gao , Junjun Shi , Jin Zhong , Huiguo Wang . Ba²⁺/Ca²⁺ co-crosslinked alginate hydrogel filtration membrane with high strength, high flux and stability for dye/salt separation. Chinese Chemical Letters, 2024, 35(5): 108820-. doi: 10.1016/j.cclet.2023.108820

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(5)
HTML views(1)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142