Citation: Qiang Luo, Jinfeng Sun, Zhibo Li, Bin Liu, Jianxun Ding. Thermo-sensitive poly(amino acid) hydrogel mediates cytoprotection through an antioxidant mechanism[J]. Chinese Chemical Letters, ;2025, 36(7): 110433. doi: 10.1016/j.cclet.2024.110433 shu

Thermo-sensitive poly(amino acid) hydrogel mediates cytoprotection through an antioxidant mechanism

Qiang Luo ^{a, b} ,
Jinfeng Sun ^b ,
Zhibo Li ^a ,
Bin Liu ^{a, *,} ,
Jianxun Ding ^{b, c, *,}

a.
Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, China
b.
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
c.
School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230026, China

lbin99@jlu.edu.cn

jxding@ciac.ac.cn

Received Date: 3 August 2024
Revised Date: 4 September 2024
Accepted Date: 8 September 2024
Available Online: 11 September 2024

Figures(5)

Oxidative stress, characterized by the excessive accumulation of reactive oxygen species (ROS), is linked to various pathological conditions, including myocardial infarction, cancer, and neurodegenerative diseases. Addressing ROS-induced cell damage has become a critical focus of biomedical research. In this study, a thermo-sensitive poly(amino acid) hydrogel, composed of poly(ethylene glycol)-block-poly(l-methionine), was prepared for cytoprotection through ROS scavenging. The sol-to-gel transition mechanism of the hydrogel was elucidated, and its potent antioxidant properties and cell protective effects were validated using hydrogen peroxide (H₂O₂)-induced oxidative stress and oxygen-glucose deprivation (OGD) models. The hydrogel significantly mitigated H₂O₂-induced damage in L929 cells, doubling their survival rate. Additionally, it scavenged approximately 35.8% of the ROS during OGD, reducing mitochondrial oxidative damage and resulting in a 29.4% decrease in apoptotic cell number. These findings underscore the potential biomedical applications of thermo-sensitive poly(amino acid) hydrogels, particularly in treating oxidative stress-related cell damage.
- Poly(amino acid),
- Thermo-sensitive hydrogel,
- Antioxidant,
- Oxidative stress,
- Cytoprotection
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