Citation: Wanpeng Zhou, Xuwen Da, Yunli Xu, Yatong Peng, Xiulian Liu, Yao Wu, Yu Shi, Aifeng Wu, Yishan Yao, Xuesong Wang, Qianxiong Zhou. HClO-responsive dinuclear Ru(Ⅱ) complexes for selective imaging and efficient photo-inactivation of intracellular bacteria[J]. Chinese Chemical Letters, ;2025, 36(6): 110376. doi: 10.1016/j.cclet.2024.110376 shu

HClO-responsive dinuclear Ru(Ⅱ) complexes for selective imaging and efficient photo-inactivation of intracellular bacteria

Wanpeng Zhou ^{a, b} ,
Xuwen Da ^a ,
Yunli Xu ^{a, b} ,
Yatong Peng ^{a, b} ,
Xiulian Liu ^{a, b} ,
Yao Wu ^{a, b} ,
Yu Shi ^{a, b} ,
Aifeng Wu ^{a, b} ,
Yishan Yao ^{c, *,} ,
Xuesong Wang ^{a, b, *,} ,
Qianxiong Zhou ^{a, *,}

a.
Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
b.
University of Chinese Academy of Sciences, Beijing 100049, China
c.
Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China

spray_yao123456@hotmail.com

xswang@mail.ipc.ac.cn

zhouqianxiong@mail.ipc.ac.cn

Received Date: 15 June 2024
Revised Date: 22 August 2024
Accepted Date: 26 August 2024
Available Online: 28 August 2024

Figures(7)

Intracellular bacteria (ICB), cloaked by the protective barriers of host cells, pose a formidable challenge to selective and efficient eradication. The employment of activatable photosensitizers based antibacterial photodynamic therapy (aPDT) holds significant potential for selective imaging and photo-inactivation of ICB while minimizing side effects on normal cells. Drawing inspiration from the elevated hypochlorous acid (HClO) levels in ICB infected phagocytes, herein we firstly designed and synthesized a series of HClO-responsive dinuclear Ru(Ⅱ) complexes (Ru1-Ru3) to achieve such a goal. Initially, the luminescence, ¹O₂ generation and aPDT activity of these Ru(Ⅱ) complexes were suppressed due to the quenching effect of the azo group, but were recovered after reaction with HClO in solutions or within ICB infected phagocytes. The detailed results revealed that Ru1 and Ru3 could not only selectively visualize ICB, but also demonstrated remarkable aPDT activity against ICB, surpassing vancomycin both in vitro and in vivo.
- Intracellular bacteria,
- HClO-responsive,
- Ru(Ⅱ) complexes,
- Antibacterial photodynamic therapy,
- Selective imaging and photo-inactivation
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