Citation: Bairui Zeng, Zhixiang Mu, Tianxi Shen, Xiaoliang Qi, Yuanqi Chen, Kezheng Lei, Chen Huang, Yi Wang, Rongdang Hu, Xiaojun Cai, Jianliang Shen, Hui Deng. A self-propelled nanovesicle with robust antibacterial and regeneration-promoting capabilities for treating biofilm-induced periodontitis[J]. Chinese Chemical Letters, ;2025, 36(6): 110350. doi: 10.1016/j.cclet.2024.110350 shu

A self-propelled nanovesicle with robust antibacterial and regeneration-promoting capabilities for treating biofilm-induced periodontitis

Bairui Zeng ^{a, 1} ,
Zhixiang Mu ^{a, 1} ,
Tianxi Shen ^a ,
Xiaoliang Qi ^{b, c} ,
Yuanqi Chen ^a ,
Kezheng Lei ^a ,
Chen Huang ^a ,
Yi Wang ^a ,
Rongdang Hu ^a ,
Xiaojun Cai ^{a, *,} ,
Jianliang Shen ^{b, c, *,} ,
Hui Deng ^{a, *,}

a.
School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou 325027, China
b.
National Engineering Research Center of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China
c.
Zhejiang Engineering Research Center for Tissue Repair Materials, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, China

cxj520118@wmu.edu.cn

shenjl@wiucas.ac.cn

dh0726@163.com

Received Date: 17 June 2024
Revised Date: 18 August 2024
Accepted Date: 19 August 2024
Available Online: 22 August 2024

Figures(5)

In the fight against bacterial infections, it is critical to effectively disrupt biofilms. However, disruption of biofilms becomes exceptionally difficult due to the low permeability of therapeutic agents. Herein, we present a self-propelled nanovesicle (PCL-PLG@CHX) strategy for eliminating biofilms and further expediting the healing of wounds. PCL-PLG@CHX is synthesized by assembling vesicles from amphiphilic polymers, which incorporate both poly-ε-caprolactone and guanidinated-poly-ε-lysine (PCL-PLG) and are infused with chlorhexidine (CHX). Upon application to sites of bacterial infection, PCL-PLG@CHX, abundant in guanidinium structures, effectively accumulates on the negatively charged surface of biofilms. It interacts with reactive oxygen species (ROS) within the biofilm, leading to nitric oxide (NO) production. The generated NO cannot only propel the nanovesicle to penetrate deeper into the biofilm, but also act as a signaling molecule to disperse the biofilm, working in conjunction with the subsequent release of CHX for an enhanced antibacterial impact. Following the eradication of bacteria, the residual guanidine component continues to produce small quantities of NO, facilitating angiogenesis and epithelial growth, thereby accelerating the healing of wounds. Together, our study shows that PCL-PLG@CHX utilizes the potential of guanidine moieties to efficiently break down biofilms and support tissue restoration, tackling the pivotal challenge of biofilm-related diseases.
- Polymer vesicles,
- Biofilm,
- Nitric oxide,
- Gas therapy,
- Periodontitis
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