Citation: Feng Shi, Guiling Li, Haibing Zhu, Ling Li, Ming Chen, Juan Li, Huifang Shen, Hao Zeng, Lingfeng Min, Zhanjun Yang. Nanozyme-triggered polymerization amplification strategy for constructing highly sensitive surface plasmon resonance immunosensing[J]. Chinese Chemical Letters, ;2025, 36(6): 110333. doi: 10.1016/j.cclet.2024.110333 shu

Nanozyme-triggered polymerization amplification strategy for constructing highly sensitive surface plasmon resonance immunosensing

Feng Shi ^{a, 1} ,
Guiling Li ^{c, 1} ,
Haibing Zhu ^{a, 1} ,
Ling Li ^a ,
Ming Chen ^a ,
Juan Li ^a ,
Huifang Shen ^a ,
Hao Zeng ^{b, *,} ,
Lingfeng Min ^{c, *,} ,
Zhanjun Yang ^{a, *,}

a.
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China
b.
National Engineering Research Center of Immunological, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy and Laboratory Medicine, Third Military Medical University, Chongqing 400037, China
c.
Department of Respiratory and Critical Care Medicine, Northern Jiangsu People’s Hospital, Clinical Medical College, Yangzhou University, Yangzhou 225001, China

zeng1109@163.com

minlingfeng@126.com

zjyang@yzu.edu.cn

Received Date: 19 May 2024
Revised Date: 1 July 2024
Accepted Date: 9 August 2024
Available Online: 14 August 2024

Figures(6)

Although diverse signal-amplified methods have been committed to improve the sensitivity of surface plasmon resonance (SPR) biosensing, introducing convenient and robust signal amplification strategy into SPR biosensing remains challenging. Here, a novel nanozyme-triggered polymerization amplification strategy was proposed for constructing highly sensitive surface plasmon resonance (SPR) immunosensor. In detail, Au@Pd core-shell nanooctahedra nanozyme with superior peroxidase (POD)-like activity was synthesized and utilized as a label probe. Simultaneously, Au@Pd core-shell nanooctahedra nanozyme can catalyze the decomposition of H₂O₂ to form hydroxyl radicals (^•OH) that triggers the polymerization of aniline to form polyaniline attaching on the surface of sensor chip, significantly amplifying SPR responses. The sensitivity of SPR immunosensor was enhanced by nanozyme-triggered polymerization amplification strategy. Using human immunoglobulin G (HIgG) as a model, the constructed SPR immunosensor obtains a wide linear range of 0.005–1.0 µg/mL with low detection limit of 0.106 ng/mL. This research provides new sights on establishing sensitive SPR immunosensor and may evokes more inspiration for developing signal amplification methods based on nanozyme in biosensing.
- Au@Pd core-shell nanooctahedra nanozyme,
- Polymerization amplification,
- POD-like activity,
- SPR immunosensor
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