Citation: Huamei Zhang, Jingjing Liu, Mingyue Li, Shida Ma, Xucong Zhou, Aixia Meng, Weina Han, Jin Zhou. Imaging polarity changes in pneumonia and lung cancer using a lipid droplet-targeted near-infrared fluorescent probe[J]. Chinese Chemical Letters, ;2024, 35(12): 110020. doi: 10.1016/j.cclet.2024.110020 shu

Imaging polarity changes in pneumonia and lung cancer using a lipid droplet-targeted near-infrared fluorescent probe

Huamei Zhang ^{a, 1} ,
Jingjing Liu ^{b, 1} ,
Mingyue Li ^a ,
Shida Ma ^a ,
Xucong Zhou ^a ,
Aixia Meng ^a ,
Weina Han ^{a, *,} ,
Jin Zhou ^{a, *,}

a.
School of Pharmacy, School of Basic Medical Sciences, Shandong Second Medical University, Weifang 261053, China
b.
Department of Respiratory and Critical Care Medicine, Weifang People's Hospital (The First Affiliated Hospital of Shandong Second Medical University), Weifang 261041, China

starhwn@sdsmu.edu.cn

zhoujin@sdsmu.edu.cn

Received Date: 7 April 2024
Revised Date: 6 May 2024
Accepted Date: 15 May 2024
Available Online: 16 May 2024

Figures(5)

As the main organ of gas exchange, the lungs are susceptible to various exogenous attacks, and pneumonia is one of the major inflammatory diseases that threaten human health. Generally, pneumonia is a disease that occurs in the alveoli and respiratory bronchioles induced by pathogens and further causes local and systemic inflammatory responses. The development of pneumonia can bring various serious complications, including lung abscess, sepsis, meningitis, brain damage and hearing loss. Over the past few decades, the mortality rate of pneumonia patients has remained high. While lung cancer is another lung-related malignant tumors worldwide, with a low 5 year survival rate. Exploring the mechanisms of their occurrence and interaction between pneumonia and lung cancer is a challenging and meaningful task. The abnormalities of lipid droplets (LDs) polarity have been found strongly accompanied by many diseases, especially cancer, inflammation, and metabolic diseases. However, their exact role is not yet clear. Hence, it is significant to develop a novel detection method to observe the polarity changes of LDs, which would help to reveal the development process of diseases pneumonia and lung cancer. In this work, a new polarity-sensitive LDs-targeted near-infrared probe BFZ up to 712 nm was designed, according to the intramolecular charge transfer mechanism, which displayed high fluorescence intensity in low polarity while showing decreased fluorescence intensity in high-polarity conditions with a significant redshift. The BFZ was successfully applied to the change of LDs polarity in lipopolysaccharide (LPS)-stimulated A549 cells, and a mouse model of lung inflammation. It also tells the polarity differences between normal and tumor cells and between normal and tumor tissues. Moreover, the correlations between pneumonia and polarity changes were observed through the imaging experiments, which may provide an insightful method for the early diagnosis of pneumonia and lung cancer.
- Fluorescent probe,
- Lipid droplets,
- Polarity,
- Pneumonia,
- Lung cancer
1. [1]
  Z. Tu, Y. Zhong, H. Hu, et al., Nat. Rev. Mater. 7 (2022) 557–574. doi: 10.1038/s41578-022-00426-z
2. [2]
  X. Zhang, W. Shu F. Tang, et al., Analyst 148 (2023) 5303–5321. doi: 10.1039/D3AN01289A
3. [3]
  M.B. Battles, J.P. Langedijk, P. Furmanova-Hollenstein, et al., Nat. Chem. Biol. 12 (2015) 87–93.
4. [4]
  G. Waterer, Breathe 17 (2021) 210087. doi: 10.1183/20734735.0087-2021
5. [5]
  H. Wang, K. Xue, P. Li, et al., Anal. Chem. 90 (2018) 6020–6027. doi: 10.1021/acs.analchem.7b04885
6. [6]
  E. Song, Y. Lai, H. Lu, et al., J. Mater. Chem. B 10 (2022) 8186–8192. doi: 10.1039/D2TB01687G
7. [7]
  Y. Ma, B. Guo, J.Y. Ge, et al., Anal. Chem. 94 (2022) 12383–12390. doi: 10.1021/acs.analchem.2c01972
8. [8]
  J. Zhang, W. Han, X. Zhou, et al., Anal. Chem. 95 (2023) 11785–11792. doi: 10.1021/acs.analchem.3c02053
9. [9]
  L. Huang, L. Zhu, W. Su, et al., Anal. Chem. 94 (2022) 11643–11649. doi: 10.1021/acs.analchem.2c02207
10. [10]
  H. Xiao, P. Li, W. Zhang, et al., Chem. Sci. 7 (2016) 1588–1593. doi: 10.1039/C5SC04099J
11. [11]
  H. Wang, M. Dong, H. Wang, et al., Chem. Commun. 57 (2021) 5838–5841. doi: 10.1039/D1CC01411K
12. [12]
  S. Wu, Y. Yan, H. Hou, et al., Anal. Chem. 94 (2022) 11238–11247. doi: 10.1021/acs.analchem.2c01737
13. [13]
  C. Liu, J. Yin, B. Lu, et al., Sens. Actuator. B: Chem. 346 (2021) 130448. doi: 10.1016/j.snb.2021.130448
14. [14]
  J. Yin, M. Peng, W. Lin, Chem. Commun. 55 (2019) 11063–11066. doi: 10.1039/C9CC04739E
15. [15]
  J. Lung, M.S. Hung, T.Y. Wang, et al., Int. J. Mol. Sci. 23 (2022) 12533. doi: 10.3390/ijms232012533
16. [16]
  M. Jiang, X. Gu, J.W.Y. Lam, et al., Chem. Sci. 8 (2017) 5440–5446. doi: 10.1039/C7SC01400G
17. [17]
  P. Ye, H. Zhang, J. Qu, et al., Org. Biomol. Chem. 20 (2022) 9184–9191. doi: 10.1039/D2OB01681H
18. [18]
  Y. Pu, R. Huang, L. Chai, et al., Sens. Actuator. B: Chem. 380 (2023) 133343. doi: 10.1016/j.snb.2023.133343
19. [19]
  C. Jin, P. Yuan, Oncol. Lett. 20 (2020) 2091–2104. doi: 10.3892/ol.2020.11769
20. [20]
  Q. Hu, W. Zhu, J. Du, et al., Chem. Commun. 59 (2023) 294–297. doi: 10.1039/D2CC06161A
21. [21]
  H. Jin, C. Ju, C. Duan, et al., Chem. Commun. 60 (2024) 1100–1103. doi: 10.1039/D3CC05529A
22. [22]
  Y. Sun, X. Zhou, L. Sun, et al., Chin. Chem. Lett. 33 (2022) 4229–4232. doi: 10.1016/j.cclet.2022.01.087
23. [23]
  Y. Liu, L. Zhang, K. Liu, et al., Chin. Chem. Lett. 35 (2024) 109759. doi: 10.1016/j.cclet.2024.109759
24. [24]
  J. Ding, R. Xiao, A. Bi, et al., Chin. Chem. Lett. 34 (2023) 108273. doi: 10.1016/j.cclet.2023.108273
25. [25]
  B. Zhu, X. Xing, J. Kim, et al., Biomaterials 304 (2024) 122419. doi: 10.1016/j.biomaterials.2023.122419
26. [26]
  Y. Pang, M. Lu, H. Rha, et al., Sci. China Chem. 67 (2023) 774–787.
27. [27]
  Y. Yang, M. Ma, L. Shen, et al., Angew. Chem. Int. Ed. 62 (2023) e202310408. doi: 10.1002/anie.202310408
28. [28]
  X. Li, X. Li, H. Ma, Chem. Sci. 11 (2019) 1617–1622.
29. [29]
  T. Zhang, F. Huo, W. Zhang, et al., Chem. Eng. J. 437 (2022) 135397. doi: 10.1016/j.cej.2022.135397
30. [30]
  L. Long, W. Liu, P. Ruan, et al., Anal. Chem. 94 (2022) 2803–2811. doi: 10.1021/acs.analchem.1c04278
31. [31]
  Y. Tang, S. Song, J. Peng, et al., J. Mater. Chem. B 10 (2022) 6974–6982. doi: 10.1039/D2TB01145J
32. [32]
  R. Chen, Z. Li, C. Peng, et al., Anal. Chem. 94 (2022) 13432–13439. doi: 10.1021/acs.analchem.2c02260
33. [33]
  R. Wang, B. Li, S. M, et al., J. Genet. Genomics 47 (2020) 69–83. doi: 10.1016/j.jgg.2019.11.009
34. [34]
  F. Amin, F. Fathi, Ž. Reiner, et al., Arch. Med. Sci. 18 (2021) 141–152.
35. [35]
  H. Chen, J. Dong, L. Yang, et al., Anal. Biochem. 668 (2023) 115114. doi: 10.1016/j.ab.2023.115114
36. [36]
  Y. Wang, X. Han, X. Zhang, et al., Analyst 145 (2020) 1389–1395. doi: 10.1039/C9AN02436K
37. [37]
  L. Sun, M. Zou, L. Du, et al., Chem. Commun. 59 (2023) 1389–12735.
38. [38]
  G. Ma, Z. Guo, X. Zhang, et al., Sens. Actuator. B: Chem. 411 (2024) 135738. doi: 10.1016/j.snb.2024.135738
1. [1]
  Xing Tian , Di Wu , Wanheng Wei , Guifu Dai , Zhanxian Li , Benhua Wang , Mingming Yu . A lipid droplets-targetable fluorescent probe for polarity detection in cells of iron death, inflammation and fatty liver tissue. Chinese Chemical Letters, 2024, 35(6): 108912-. doi: 10.1016/j.cclet.2023.108912
2. [2]
  Han-Min Wang , Yan-Chen Li , Lu-Lu Sun , Ming-Ye Tang , Jia Liu , Jiahao Cai , Lei Dong , Jia Li , Yi Zang , Hai-Hao Han , Xiao-Peng He . Protein-encapsulated long-wavelength fluorescent probe hybrid for imaging lipid droplets in living cells and mice with non-alcoholic fatty liver. Chinese Chemical Letters, 2024, 35(11): 109603-. doi: 10.1016/j.cclet.2024.109603
3. [3]
  Fan Zheng , Runsha Xiao , Shuai Huang , Zhikang Chen , Chen Lai , Anyao Bi , Heying Yao , Xueping Feng , Zihua Chen , Wenbin Zeng . Accurate visualization colorectal cancer by monitoring viscosity variations with a novel mitochondria-targeted fluorescent probe. Chinese Chemical Letters, 2025, 36(2): 109876-. doi: 10.1016/j.cclet.2024.109876
4. [4]
  Haiyan Lu , Jiayue Ye , Yiping Wei , Hua Zhang , Konstantin Chingin , Vladimir Frankevich , Huanwen Chen . Tracing molecular margins of lung cancer by internal extractive electrospray ionization mass spectrometry. Chinese Chemical Letters, 2025, 36(2): 110077-. doi: 10.1016/j.cclet.2024.110077
5. [5]
  Lishan Xiong , Xinyuan Li , Xiaojie Lu , Zhendong Zhang , Yan Zhang , Wen Wu , Chenhui Wang . Inhaled multilevel size-tunable, charge-reversible and mucus-traversing composite microspheres as trojan horse: Enhancing lung deposition and tumor penetration. Chinese Chemical Letters, 2024, 35(9): 109384-. doi: 10.1016/j.cclet.2023.109384
6. [6]
  Yudi Cheng , Xiao Wang , Jiao Chen , Zihan Zhang , Jiadong Ou , Mengyao She , Fulin Chen , Jianli Li . A near-infrared fluorescent probe for visualizing transformation pathway of Cys/Hcy and H₂S and its applications in living system. Chinese Chemical Letters, 2024, 35(5): 109156-. doi: 10.1016/j.cclet.2023.109156
7. [7]
  Chuan-Zhi Ni , Ruo-Ming Li , Fang-Qi Zhang , Qu-Ao-Wei Li , Yuan-Yuan Zhu , Jie Zeng , Shuang-Xi Gu . A chiral fluorescent probe for molecular recognition of basic amino acids in solutions and cells. Chinese Chemical Letters, 2024, 35(10): 109862-. doi: 10.1016/j.cclet.2024.109862
8. [8]
  Tao Liu , Xuwei Han , Xueyi Sun , Weijie Zhang , Ke Gao , Runan Min , Yuting Tian , Caixia Yin . An activated fluorescent probe to monitor NO fluctuation in Parkinson’s disease. Chinese Chemical Letters, 2025, 36(3): 110170-. doi: 10.1016/j.cclet.2024.110170
9. [9]
  Zhixiao Xiong , Shanni Qiu , Yuyu Wang , Houna Duan , Yi Xiao , Yufang Xu , Weiping Zhu , Xuhong Qian . Photocalibrated NO release from the zinc ion fluorescent probe based on naphthalimide and its application in living cells. Chinese Chemical Letters, 2025, 36(4): 110002-. doi: 10.1016/j.cclet.2024.110002
10. [10]
  Chuanfeng Fan , Jian Gao , Yingkai Gao , Xintong Yang , Gaoning Li , Xiaochun Wang , Fei Li , Jin Zhou , Haifeng Yu , Yi Huang , Jin Chen , Yingying Shan , Li Chen . A non-peptide-based chymotrypsin-targeted long-wavelength emission fluorescent probe with large Stokes shift and its application in bioimaging. Chinese Chemical Letters, 2024, 35(10): 109838-. doi: 10.1016/j.cclet.2024.109838
11. [11]
  Lei Shen , Hongmei Liu , Ming Jin , Jinchao Zhang , Caixia Yin , Shuxiang Wang , Yutao Yang . “Three-in-one” strategy of trifluoromethyl regulated blood-brain barrier permeable fluorescent probe for peroxynitrite and antiepileptic evaluation of edaravone. Chinese Chemical Letters, 2024, 35(10): 109572-. doi: 10.1016/j.cclet.2024.109572
12. [12]
  Lixian Fu , Yiyun Tan , Yue Ding , Weixia Qing , Yong Wang . Water–soluble and polarity–sensitive near–infrared fluorescent probe for long–time specific cancer cell membranes imaging and C. Elegans label. Chinese Chemical Letters, 2024, 35(4): 108886-. doi: 10.1016/j.cclet.2023.108886
13. [13]
  Jiajia Lv , Jie Gao , Hongyu Li , Zeli Yuan , Nan Dong . Rational design of hydroxytricyanopyrrole-based probes with high affinity and rapid visualization for amyloid-β aggregates in vitro and in vivo. Chinese Chemical Letters, 2024, 35(5): 108940-. doi: 10.1016/j.cclet.2023.108940
14. [14]
  Chao Liu , Chao Jia , Shi-Xian Gan , Qiao-Yan Qi , Guo-Fang Jiang , Xin Zhao . A luminescent one-dimensional covalent organic framework for organic arsenic sensing in water. Chinese Chemical Letters, 2024, 35(11): 109750-. doi: 10.1016/j.cclet.2024.109750
15. [15]
  Yunkang Tong , Haiqiao Huang , Haolan Li , Mingle Li , Wen Sun , Jianjun Du , Jiangli Fan , Lei Wang , Bin Liu , Xiaoqiang Chen , Xiaojun Peng . Cooperative bond scission by HRP/H₂O₂ for targeted prodrug activation. Chinese Chemical Letters, 2024, 35(12): 109663-. doi: 10.1016/j.cclet.2024.109663
16. [16]
  Quan Zhang , Shunjie Xing , Jingqian Han , Li Feng , Jianchun Li , Zhaosheng Qian , Jin Zhou . Organic pollutant sensing for human health based on carbon dots. Chinese Chemical Letters, 2025, 36(1): 110117-. doi: 10.1016/j.cclet.2024.110117
17. [17]
  Hui Zhang , Rong Feng , Wanyi Yu , Hongbei Wei , Tianhong Wu , Peng Zhang , Wenhai Bian , Xin Li , Di Gao , Guojun Weng , Zhe Yang , Tony D. James , Xiaolong Sun . Evaluating the global thiols redox state in living cells using a reducing sulfur species responsive fluorescence switching platform. Chinese Chemical Letters, 2025, 36(4): 110528-. doi: 10.1016/j.cclet.2024.110528
18. [18]
  Wei-Tao Dou , Qing-Wen Zeng , Yan Kang , Haidong Jia , Yulian Niu , Jinglong Wang , Lin Xu . Construction and application of multicomponent fluorescent droplets. Chinese Chemical Letters, 2025, 36(1): 109995-. doi: 10.1016/j.cclet.2024.109995
19. [19]
  Zhi Li , Shuya Pan , Yuan Tian , Shaowei Liu , Weifeng Wei , Jinlin Wang , Tianfeng Chen , Ling Wang . Selenium nanoparticles enhance the chemotherapeutic efficacy of pemetrexed against non-small cell lung cancer. Chinese Chemical Letters, 2024, 35(12): 110018-. doi: 10.1016/j.cclet.2024.110018
20. [20]
  Lingjun Sha , Bing Bo , Jiayu Li , Qi Liu , Ya Cao , Jing Zhao . Precise assessment of lung cancer-derived exosomes based on dual-labelled membrane interface. Chinese Chemical Letters, 2025, 36(4): 110109-. doi: 10.1016/j.cclet.2024.110109

Get Citation

PDF

XML

Metrics

PDF Downloads(5)
Abstract views(347)
HTML views(16)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142