Citation: Xu Qu, Pengzhao Wu, Kaixuan Duan, Guangwei Wang, Liang-Liang Gao, Yuan Guo, Jianjian Zhang, Donglei Shi. Self-calibrating probes constructed on a unique dual-emissive fluorescence platform for the precise tracking of cellular senescence[J]. Chinese Chemical Letters, ;2024, 35(12): 109681. doi: 10.1016/j.cclet.2024.109681 shu

Self-calibrating probes constructed on a unique dual-emissive fluorescence platform for the precise tracking of cellular senescence

Xu Qu ^{a, 1} ,
Pengzhao Wu ^{a, 1} ,
Kaixuan Duan ^{a, 1} ,
Guangwei Wang ^a ,
Liang-Liang Gao ^a ,
Yuan Guo ^{a, *,} ,
Jianjian Zhang ^{a, *,} ,
Donglei Shi ^{a, b, *,}

a.
Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, China
b.
Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Pharmaceutical Sciences, Hainan University, Haikou 570228, China

guoyuan@nwu.edu.cn

zhangjj@nwu.edu.cn

dongleishi@hainanu.edu.cn

Received Date: 20 December 2023
Revised Date: 19 February 2024
Accepted Date: 21 February 2024
Available Online: 5 March 2024

Figures(4)

Human β-galactosidase (β-gal) is recognized as a crucial biomarker for evaluating senescence at the cellular and tissue levels in humans. However, tools to precisely track the endogenous β-gal are still limited. Herein, we present two novel self-calibrating β-gal probes 7a and 7b which were constructed on a unique green/red dual-emissive fluorescence platform. The two probes inherently exhibited a stable green fluorescence signal impervious to β-gal activity, serving as a reliable internal reference. They also displayed a progressively diminishing red fluorescence signal with the increasing of β-gal expression levels. The dual behavior endows them with self-calibration capacity and then renders excellently selective and sensitive for precisely monitoring β-gal activity. Notably, compared with E. coli β-gal, the two probes are more effectively response to A. oryzae β-gal homologous to human β-gal, indicating their unique species-selectivity. Furthermore, 7a was validated for its effectiveness in determining senescence-associated β-galactosidase (SA-β-gal) expression in senescent NRK-52E and HepG2 cells, underscoring its practical applicability in senescence research.
- Senescence associated β-galactosidase,
- Dual-emission,
- Self-calibration,
- Cell imaging,
- Fluorescent probes
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