Citation: Yunan Yuan, Zhimin Luo, Jie Chen, Chaoliang He, Kai Hao, Huayu Tian. Constructing thermoresponsive PNIPAM-based microcarriers for cell culture and enzyme-free cell harvesting[J]. Chinese Chemical Letters, ;2024, 35(7): 109549. doi: 10.1016/j.cclet.2024.109549 shu

Constructing thermoresponsive PNIPAM-based microcarriers for cell culture and enzyme-free cell harvesting

Yunan Yuan ^{a, b, d} ,
Zhimin Luo ^{c, e} ,
Jie Chen ^{a, d} ,
Chaoliang He ^{a, b} ,
Kai Hao ^{c, *,} ,
Huayu Tian ^{a, b, c, d, e, *,}

a.
CAS Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
b.
School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230026, China
c.
College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
d.
Jilin Biomedical Polymers Engineering Laboratory, Changchun 130022, China
e.
Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen 361005, China

kaihao@ciac.ac.cn

thy@xmu.edu.cn

Received Date: 14 November 2023
Revised Date: 17 January 2024
Accepted Date: 19 January 2024
Available Online: 23 January 2024

Figures(4)

The development of large-scale cell cultivation and non-invasive cell harvesting is highly desired in various fields, including biological regeneration and pharmaceutical research. When using traditional microcarriers for cell culture, trypsinization is often necessary during cell collection, leading to partial cells damage. In this work, we developed a thermoresponsive glass microcarrier modified with poly(γ-propargyl-ʟ-glutamate) (PPLG) and poly(N-isopropylacrylamide) (PNIPAM). We utilized these microcarriers for three-dimensional cell culture and enzyme-free cell harvesting, and the results indicated that the prepared microcarriers exhibited excellent non-invasive cell culture performance.
- Microcarrier,
- Temperature-responese,
- PNIPAM,
- Polymers,
- Cell culture
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