Citation: Yanqi Wu, Yuhong Guan, Peilin Huang, Hui Chen, Liping Bai, Zhihong Jiang. Preparation of norovirus GII loop mediated isothermal amplification freeze-drying microsphere reagents and its application in an on-site integrated rapid detection platform[J]. Chinese Chemical Letters, ;2024, 35(9): 109308. doi: 10.1016/j.cclet.2023.109308 shu

Preparation of norovirus GII loop mediated isothermal amplification freeze-drying microsphere reagents and its application in an on-site integrated rapid detection platform

Yanqi Wu ^{a, b, 1} ,
Yuhong Guan ^{c, 1} ,
Peilin Huang ^b ,
Hui Chen ^c ,
Liping Bai ^a ,
Zhihong Jiang ^{a, *,}

a.
State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macao 999078, China
b.
Shenzhen LemnisCare Medical Technology Co., Ltd., Shenzhen 518107, China
c.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412077, China

zhjiang@must.edu.mo

Received Date: 8 September 2023
Revised Date: 11 November 2023
Accepted Date: 14 November 2023
Available Online: 21 November 2023

Figures(4)

Norovirus is an infectious disease that can cause non-bacterial gastroenteritis, which has a low infectious dose, rapid onset, and strong transmission ability; therefore, rapid and sensitive detection is essential to reduce the transmission of gastroenteritis. In the study, a norovirus GII loop-mediated isothermal amplification assay was developed and prepared into freeze-drying microspheres, and a closed-cassette-based, integrated, reagent-ambient storage, on-site instant detection platform for norovirus GII was constructed using a commercial, fully automated nucleic acid analyzer with integrated magnetic bearing based nuclear acid extraction and nucleic acid detection, with a sensitivity of 10 copies/µL, with no cross-reactivity with other 5 viruses. For 28 simulated samples, the integrated assay platform was consistent with the experimental results of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays after conventional laboratory nucleic acid extraction. The entire process can be finished in about 1 h, which is ideal for immediate rapid detection.
- Freeze-dried microspheres,
- Point-of-care testing,
- Loop-mediated isothermal amplification,
- Norovirus GII,
- Integrated testing
1. [1]
  A.N. Desai, JAMA 322 (2019) 2032. doi: 10.1001/jama.2019.15921
2. [2]
  N. Dabilla, T.N. Vieira Almeida, A.C. Reboucas Oliveira, et al., J. Clin. Virol. 87 (2017) 60–66. doi: 10.1016/j.jcv.2016.12.009
3. [3]
  G.A.M. Tarr, X.L. Pang, R. Zhuo, et al., J. Infect. Dis. 223 (2021) 452–461. doi: 10.1093/infdis/jiaa391
4. [4]
  C. Dongqing, Z. Songyan, L. Ningbo, et al., J. Food Sci. 83 (2018) 393–400. doi: 10.1111/1750-3841.14022
5. [5]
  P. Chhabra, H. Browne, T. Huynh, et al., J. Clin. Virol. 134 (2021) 104689. doi: 10.1016/j.jcv.2020.104689
6. [6]
  S. Honjo, K. Kuronuma, Y. Fujiya, et al., Infect. Genet. Evol. 104 (2022) 105348. doi: 10.1016/j.meegid.2022.105348
7. [7]
  M. Farahmand, M. Moghoofei, A. Dorost, et al., Rev. Med. Virol. 32 (2022) e2237. doi: 10.1002/rmv.2237
8. [8]
  R.M. Callejon, M. Isabel Rodriguez-Naranjo, C. Ubeda, et al., Foodborne Pathog. Dis. 12 (2015) 32–38. doi: 10.1089/fpd.2014.1821
9. [9]
  Z. Chen, T. Yang, H. Yang, et al., J. Biomed. Nanotechnol. 14 (2018) 198–205. doi: 10.1166/jbn.2018.2524
10. [10]
  K. Leera, T. Anyarat, C. Suwat, et al., Food Environ. Virol. 8 (2016) 133–140. doi: 10.1007/s12560-016-9228-6
11. [11]
  C. Tang, Z. He, H. Liu, et al., J. Nanobiotechnol. 18 (2020) 62. doi: 10.1186/s12951-020-00613-6
12. [12]
  Y. Fang, H. Liu, Y. Wang, et al., J. Biomed. Nanotechnol. 17 (2021) 407–415. doi: 10.1166/jbn.2021.3028
13. [13]
  Y. Yongxin, C. Hui, H. Linghao, et al., Appl. Environ. Microbiol. 81 (2015) 7615–7624. doi: 10.1128/AEM.01729-15
14. [14]
  L. He, H. Yang, P. Xiao, et al., J. Biomed. Nanotechnol. 13 (2017) 1243–1252. doi: 10.1166/jbn.2017.2422
15. [15]
  H. Chen, Y. Wu, Z. Chen, et al., J. Biomed. Nanotechnol. 13 (2017) 1619–1630. doi: 10.1166/jbn.2017.2478
16. [16]
  N.O. Prado, L.A. Lalli, L. Blanes, D.L. Zanette, M.N. Aoki, Mini Rev. Med. Chem. 23 (2023) 480–496. doi: 10.2174/1389557522666220802144057
17. [17]
  L.J.J. Hansen, R. Daoussi, C. Vervaet, J.P. Remon, T.R.M. De Beer, Vaccine 33 (2015) 5507–5519. doi: 10.1016/j.vaccine.2015.08.085
18. [18]
  A. Ahlford, B. Kjeldsen, J. Reimers, et al., Analyst 135 (2010) 2377–2385. doi: 10.1039/c0an00321b
19. [19]
  D. Fissore, T. McCoy, Front. Chem. 6 (2018) 622. doi: 10.3389/fchem.2018.00622
20. [20]
  L. Thirion, A. Dubot-Peres, L. Pezzi, et al., Viruses 12 (2020) 159. doi: 10.3390/v12020159
21. [21]
  X. Zhang, Y. Chen, Y. Pan, et al., Chin. Chem. Lett. 35 (2024) 108378. doi: 10.1016/j.cclet.2023.108378
22. [22]
  S.B. Jeon, D.J. Seo, H. Oh, D.H. Kingsley, C. Choi, Food Control 73 (2017) 1002–1009. doi: 10.1016/j.foodcont.2016.10.005
23. [23]
  X.F. Qian, A.L. Duan, R.X. Huang, et al., J. Clin. Lab. Anal. 35 (2021) e23919. doi: 10.1002/jcla.23919
24. [24]
  Y. Pan, Y. Xiao, Y. Hao, et al., Chin. Chem. Lett. 33 (2022) 2486–2490. doi: 10.1016/j.cclet.2021.12.093
1. [1]
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2. [2]
  Wei-Yu Zhou , Zi-Han Xi , Ning-Ning Du , Li Ye , Ming-Hao Jiang , Jin-Le Hao , Bin Lin , Guo-Dong Yao , Xiao-Xiao Huang , Shao-Jiang Song . Rapid discovery of two unprecedented meroterpenoids from Daphne altaica Pall. using molecular networking integrated with MolNetEnhancer and Network Annotation Propagation. Chinese Chemical Letters, 2024, 35(8): 109030-. doi: 10.1016/j.cclet.2023.109030
3. [3]
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4. [4]
  Ya Song , Mingxia Zhou , Zhu Chen , Huali Nie , Jiao-Jing Shao , Guangmin Zhou . Integrated interconnected porous and lamellar structures realized fast ion/electron conductivity in high-performance lithium-sulfur batteries. Chinese Chemical Letters, 2024, 35(6): 109200-. doi: 10.1016/j.cclet.2023.109200
5. [5]
  Xiaoliu Liang , Chunliu Huang , Hui Liu , Hu Chen , Jiabao Shou , Hongwei Cheng , Gang Liu . Natural hydrogel dressings in wound care: Design, advances, and perspectives. Chinese Chemical Letters, 2024, 35(10): 109442-. doi: 10.1016/j.cclet.2023.109442
6. [6]
  Xueyan Zhang , Jicong Chen , Songren Han , Shiyan Dong , Huan Zhang , Yuhong Man , Jie Yang , Ye Bi , Lesheng Teng . The size-switchable microspheres co-loaded with RANK siRNA and salmon calcitonin for osteoporosis therapy. Chinese Chemical Letters, 2024, 35(12): 109668-. doi: 10.1016/j.cclet.2024.109668
7. [7]
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8. [8]
  Rui Li , Ruijie Lu , Libin Yang , Jianwen Li , Zige Guo , Qiquan Yan , Mengjun Li , Yazhuo Ni , Keying Chen , Yaoyang Li , Bo Xu , Mengzhen Cui , Zhan Li , Zhiying Zhao . Immobilization of chitosan nano-hydroxyapatite alendronate composite microspheres on polyetheretherketone surface to enhance osseointegration by inhibiting osteoclastogenesis and promoting osteogenesis. Chinese Chemical Letters, 2025, 36(4): 110242-. doi: 10.1016/j.cclet.2024.110242
9. [9]
  Lishan Xiong , Xinyuan Li , Xiaojie Lu , Zhendong Zhang , Yan Zhang , Wen Wu , Chenhui Wang . Inhaled multilevel size-tunable, charge-reversible and mucus-traversing composite microspheres as trojan horse: Enhancing lung deposition and tumor penetration. Chinese Chemical Letters, 2024, 35(9): 109384-. doi: 10.1016/j.cclet.2023.109384
10. [10]
  Ruixin Liu , Feng Shi , Yanping Xia , Haibing Zhu , Jiawen Cao , Kai Peng , Chuanli Ren , Juan Li , Zhanjun Yang . Universal MOF nanozyme-induced catalytic amplification strategy for label-free electrochemical immunoassay. Chinese Chemical Letters, 2024, 35(11): 109664-. doi: 10.1016/j.cclet.2024.109664
11. [11]
  Qinghong Pan , Huafang Zhang , Qiaoling Liu , Donghong Huang , Da-Peng Yang , Tianjia Jiang , Shuyang Sun , Xiangrong Chen . A self-powered cathodic molecular imprinting ultrasensitive photoelectrochemical tetracycline sensor via ZnO/C photoanode signal amplification. Chinese Chemical Letters, 2025, 36(1): 110169-. doi: 10.1016/j.cclet.2024.110169
12. [12]
  Bo Liu , Shuaiqiang Shao , Junjie Cai , Zijian Zhang , Feng Tian , Kun Yang , Fan Li . Signal cascade amplification of streptavidin-biotin-modified immunofluorescence nanocapsules for ultrasensitive detection of glial fibrillary acidic protein. Chinese Chemical Letters, 2025, 36(3): 109814-. doi: 10.1016/j.cclet.2024.109814
13. [13]
  Yuan Dong , Mutian Ma , Zhenyang Jiao , Sheng Han , Likun Xiong , Zhao Deng , Yang Peng . Effect of electrolyte cation-mediated mechanism on electrocatalytic carbon dioxide reduction. Chinese Chemical Letters, 2024, 35(7): 109049-. doi: 10.1016/j.cclet.2023.109049
14. [14]
  Zhuangzhuang Zhang , Yaru Qiao , Jun Zhao , Dai-Huo Liu , Mengmin Jia , Hongwei Tang , Liang Wang , Dongmei Dai , Bao Li . Fluorine-doped K_0.39Mn_0.77Ni_0.23O_1.9F_0.1 microspheres with highly reversible oxygen redox reaction for potassium-ion battery cathode. Chinese Chemical Letters, 2025, 36(3): 109907-. doi: 10.1016/j.cclet.2024.109907
15. [15]
  Sixin Ai , Wenxiu Li , Huayong Zhu , Yang Wan , Weiying Lin . Viscosity-responsive signal amplification dual-modal probe triggered by cysteine/homocysteine for monitoring diabetic liver damages and repair processes. Chinese Chemical Letters, 2025, 36(3): 109904-. doi: 10.1016/j.cclet.2024.109904
16. [16]
  Ruiheng Liang , Huizhong Wu , Zhongzheng Hu , Ge Song , Xuyang Zhang , Omotayo A. Arotiba , Minghua Zhou . Hierarchical Fe-Bi/Bi₇O₉I₃/OVs microspheres coupled with natural air diffusion electrode to achieve efficient heterogeneous visible-light-driven photoelectro-Fenton degradation of tetracycline without aeration. Chinese Chemical Letters, 2025, 36(4): 110136-. doi: 10.1016/j.cclet.2024.110136
17. [17]
  Wen Xiao , Fazhan Wang , Yangzhuo Gu , Xi He , Na Fan , Qian Zheng , Shugang Qin , Zhongshan He , Yuquan Wei , Xiangrong Song . PEG400-mediated nanocarriers improve the delivery and therapeutic efficiency of mRNA tumor vaccines. Chinese Chemical Letters, 2024, 35(5): 108755-. doi: 10.1016/j.cclet.2023.108755
18. [18]
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19. [19]
  Yiqian Jiang , Zihan Yang , Xiuru Bi , Nan Yao , Peiqing Zhao , Xu Meng . Mediated electron transfer process in α-MnO₂ catalyzed Fenton-like reaction for oxytetracycline degradation. Chinese Chemical Letters, 2024, 35(8): 109331-. doi: 10.1016/j.cclet.2023.109331
20. [20]
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