Citation: Ling Yang, Min Ren, Jie Wang, Liming He, Shanshan Wu, Shuai Yang, Wei Zhao, Hao Cheng, Xiaoming Zhou, Maling Gou. A non-viral gene therapy for melanoma by staphylococcal enterotoxin A[J]. Chinese Chemical Letters, ;2024, 35(5): 108822. doi: 10.1016/j.cclet.2023.108822 shu

A non-viral gene therapy for melanoma by staphylococcal enterotoxin A

Ling Yang ^{a, 1} ,
Min Ren ^{a, 1} ,
Jie Wang ^a ,
Liming He ^a ,
Shanshan Wu ^b ,
Shuai Yang ^a ,
Wei Zhao ^a ,
Hao Cheng ^a ,
Xiaoming Zhou ^c ,
Maling Gou ^{a, *,}

a.
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China
b.
Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China
c.
State Key Laboratory of Biotherapy, Department of Integrated Traditional Chinese and Western Medicine, Rare Diseases Center, West China Hospital, Sichuan University, Chengdu 610041, China

goumaling@scu.edu.cn

Received Date: 25 May 2023
Revised Date: 11 July 2023
Accepted Date: 17 July 2023
Available Online: 20 July 2023

Figures(5)

Staphylococcal enterotoxin A (SEA) derived from Staphylococcus aureus, as a superantigen, shows potential for cancer immunotherapy, but systemic immunotoxicity restricts its clinical application. Targeted delivery of SEA to tumor site provides a promising option for reducing the systemic toxicity. Here, we constructed an iRGD peptide (H-[Cys-Arg-Gly-Asp-Lys-Gly-Pro-Asp-Cys]-NH₂) modified nanoparticle (iDPP) to deliver plasmids encoding SEA for melanoma treatment. The iDPP/SEA nanocomplexes efficiently mediated SEA expression in B16-F10 cells in vivo and in vitro and induced the activation of lymphocytes and maturation of murine bone marrow-derived dendritic cells (BMDCs) in vitro. In the subcutaneous B16-F10 melanoma model, the iDPP/SEA nanocomplexes could effectively enhance immune response and T lymphocytes infiltration in tumor site after intravenous administration, thereby considerably decreased melanoma growth. Meanwhile, no obvious adverse effect was observed after intravenous administration of the iDPP/SEA nanocomplexes in vivo. Our findings demonstrated that gene therapy of SEA is a potential candidate for melanoma treatment.
- Gene therapy,
- Superantigen,
- Melanoma,
- Staphylococcal enterotoxins A,
- Immunotherapyene therapy
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