Citation: Haiting Pan, Xianzhi Chai, Junji Zhang. A near-infrared fluorescent probe for fast and precise imaging of senescent cells and ovarian cancer cells via tracking β-galactosidase[J]. Chinese Chemical Letters, ;2023, 34(12): 108321. doi: 10.1016/j.cclet.2023.108321 shu

A near-infrared fluorescent probe for fast and precise imaging of senescent cells and ovarian cancer cells via tracking β-galactosidase

Haiting Pan ^a ,
Xianzhi Chai ^{b, *,} ,
Junji Zhang ^{a, *,}

a.
Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, Frontiers Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, Shanghai 200237, China
b.
School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212100, China

chaixianzhi@163.com

zhangjunji@ecust.edu.cn

Received Date: 21 December 2022
Revised Date: 1 March 2023
Accepted Date: 8 March 2023
Available Online: 11 March 2023

Figures(5)

Aging-related diseases are gradually becoming a major problem with the rapid development of aged population in human society. Although many fluorescent probes have been employed to diagnosis senescence via imaging senescence-associated β-galactosidase (SA-β-Gal), which is proved to be closely associated with senescent cells, the similar catalytic effectiveness of enzymatic reaction of ovarian cancer-associated β-Gal (OA-β-Gal) will interfere with imaging accuracy. Herein, a near-infrared (NIR) hemicyanine based fluorescent probe HCyXA-βGal was designed for light-up imaging of live cells containing β-Gal. With the organelle-targeting morpholinyl and positive charge moieties, HCyXA-βGal was successfully applicated to image the difference of enzymatic location in senescent cells and ovarian cancer cells. Furthermore, inspired by the fast response performance, fast and precise imaging of the two cell lines was realized via covering another dimension of fluorescence signal: time-dependent intensity.
- β-Galactosidase,
- Near-infrared fluorescent probe,
- Senescent cell,
- Precise imaging,
- Fast response
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