Citation: Yangwode Jing, Chong Zuo, Yun-Xiang Du, Junxiong Mao, Ruichao Ding, Jiachen Zhang, Lu-Jun Liang, Qian Qu. Chemical tools for E3 ubiquitin ligase study[J]. Chinese Chemical Letters, ;2023, 34(4): 107781. doi: 10.1016/j.cclet.2022.107781 shu

Chemical tools for E3 ubiquitin ligase study

Yangwode Jing ^{a, b} ,
Chong Zuo ^b ,
Yun-Xiang Du ^b ,
Junxiong Mao ^b ,
Ruichao Ding ^b ,
Jiachen Zhang ^b ,
Lu-Jun Liang ^b ,
Qian Qu ^{a, *,}

a.
Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China
b.
Tsinghua-Peking Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Center for Synthetic and Systems Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China

quqian22@sjtu.edu.cn

Received Date: 14 June 2022
Revised Date: 17 August 2022
Accepted Date: 23 August 2022
Available Online: 28 August 2022

Figures(9)

E3 ubiquitin ligases catalyze the final step of ubiquitylation, a crucial post-translational modification involved in almost every process in eukaryotic cells. E3 ubiquitin ligases are key regulators of cellular events, and the investigation into their functions and functioning mechanisms are research areas with great importance. Synthetic or semi-synthetic tools have greatly facilitated the research about the enzyme activity, distribution in different physiological events, and catalytic mechanism of E3 ubiquitin ligase. In this review, we summarize the development of chemical tools for E3 ubiquitin ligases with an emphasis on the synthetic routes. We show the utility of these chemical tools by briefly discussing their applications in biological research.
- Chemical tools,
- Protein chemical synthesis,
- Ubiquitylation,
- E3 ligase,
- Enzymatic mechanism
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