Citation: Peng Shen, Han Lin, Yikai Bao, Haofei Hong, Zhimeng Wu. Synthesis and immunological study of a glycosylated wall teichoic acid-based vaccine against Staphylococcus aureus[J]. Chinese Chemical Letters, ;2023, 34(4): 107679. doi: 10.1016/j.cclet.2022.07.022 shu

Synthesis and immunological study of a glycosylated wall teichoic acid-based vaccine against Staphylococcus aureus

Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China

zhimengw@hotmail.com

Received Date: 11 May 2022
Revised Date: 5 July 2022
Accepted Date: 12 July 2022
Available Online: 14 July 2022

Figures(6)

Staphylococcus aureus wall teichoic acids (WTAs) are attractive targets for antibacterial vaccine development. In this study, three core glycosylated WTA structure, including α-1,4-GlcNAc, β-1,4-GlcNAc and β-1,3-GlcNAc modified ribitol phosphates containing a linker are chemically synthesized and conjugated with tetanus toxin (TT) carrier protein as vaccine candidates. In vivo immunological studies demonstrate that the synthesized glycosylated WTAs display high immunogenicity and all conjugates provoke strong immune responses and elicit high levels of specific IgG antibodies against the GlcNAc-modified WTA. Furthermore, antibodies elicited by the vaccine candidates remain the capability to recognize S. aureus cells and display significant opsonophagocytic activity to clear S. aureus. This study demonstrates that the core structure of glycosylated WTAs are effective antigens for constructing anti-S. aureus vaccines to prevent and control S. aureus infections.
- WTAs,
- Staphylococcus aureus,
- GlcNAc modifications,
- Glycoconjugates,
- Antibacterial vaccine
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沈阳化工大学材料科学与工程学院沈阳 110142