Citation: Donghui Wu, Qilin Zhao, Jian Sun, Xiurong Yang. Fluorescence immunoassay based on alkaline phosphatase-induced in situ generation of fluorescent non-conjugated polymer dots[J]. Chinese Chemical Letters, ;2023, 34(3): 107672. doi: 10.1016/j.cclet.2022.07.015 shu

Fluorescence immunoassay based on alkaline phosphatase-induced in situ generation of fluorescent non-conjugated polymer dots

Donghui Wu ^{a, b} ,
Qilin Zhao ^{a, b} ,
Jian Sun ^{a, *,} ,
Xiurong Yang ^{a, b, *,}

a.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
b.
School of Applied of Chemistry and Engineering, University of Science and Technology of China, Hefei 230026, China

jiansun@ciac.ac.cn

xryang@ciac.ac.cn

Received Date: 7 April 2022
Revised Date: 22 June 2022
Accepted Date: 8 July 2022
Available Online: 9 July 2022

Figures(4)

Alkaline phosphatase (ALP) activity assay is not only significant to the clinical diagnosis of some related disease, but also momentous to the construction of ALP-based enzyme-linked immunosorbent assay (ELISA). Herein, for the first time, we have discovered that ascorbic acid (AA) can specially react with N-methylethylenediamine (N-MEDA) to generate fluorescent non-conjugated polymer dots (NCPDs) under mild conditions. On the basis of the AA-responsive emission and ALP-catalyzed hydrolysis of ascorbic acid 2-phosphate (AA2P) to AA, we have exploited a fluorometric ALP activity assay with high sensitivity and selectivity. Furthermore, by means of conventional ALP-based ELISA platform, a conceptual fluorescent ELISA has been constructed and applied in the potential clinical diagnosis, during which cardiac troponin I (cTnI), a well-established biomarker of acute myocardial infarction, has been chosen as the model target. We envision that such original fluorescent NCPDs generation-enabled ELISA could become a versatile tool in biochemical sensing and medical diagnosis in the future.
- Alkaline phosphatase,
- Non-conjugated polymer dots,
- Fluorescent sensor,
- Fluorescent ELISA,
- Cardiac troponin I
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